Influencing Factors on the Oncuria™ Urinalysis Assay: An Experimental Model.
Influencing Factors on the Oncuria™ Urinalysis Assay: An Experimental Model.
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DOI：
10.3390/diagnostics11061023
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发表时间：
2021-06-03
期刊：
影响因子：
--
通讯作者：
Furuya H
中科院分区：
文献类型：
--
作者：
Murakami K;Pagano I;Chen R;Sun Y;Goodison S;Rosser CJ;Furuya H
Background: The Oncuria™ urine test for the detection of bladder cancer measures a multiplex protein signature. In this study, we investigated the influence of urinary cellularity, protein, and hematuria on the performance of the Oncuria™ test in an ex vivo experimental model. Materials and Methods: Pooled urine from healthy subjects was spiked with cultured benign (UROtsa) or malignant cells (T24), cellular proteins, or whole blood. The resulting samples were analyzed using the Oncuria™ test following the manufacturer’s instructions. Results: Urine samples obtained from healthy subjects were negative for bladder cancer by Oncuria™ test criteria. The majority of the manipulated conditions did not result in a false-positive test. The addition of whole blood (high concentration) did result in a false-positive result, but this was abrogated by sample centrifugation prior to analysis. The addition of cellular proteins (high concentration) resulted in a positive Oncuria™ test, and this was unaffected by pre-analysis sample centrifugation. Conclusions: The Oncuria™ multiplex test performed well in the ex vivo experimental model and shows promise for clinical application. The identification of patients who require additional clinical evaluation could reduce the need to subject patients who do not have bladder cancer to frequent, uncomfortable and expensive cystoscopic examinations, thus benefiting both patients and the healthcare system.
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影响因子：
--
作者：
Chan OTM;Furuya H;Pagano I;Shimizu Y;Hokutan K;Dyrskjøt L;Jensen JB;Malmstrom PU;Segersten U;Janku F;Rosser CJ
通讯作者：
Rosser CJ
DOI：
10.1158/1055-9965.epi-08-1002
发表时间：
2009-02
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
--
作者：
Rosser CJ;Liu L;Sun Y;Villicana P;McCullers M;Porvasnik S;Young PR;Parker AS;Goodison S
通讯作者：
Goodison S
影响因子：
3.4
作者：
Faiena, Izak;Rosser, Charles J.;Furuya, Hideki
通讯作者：
Furuya, Hideki
影响因子：
120.7
作者：
Grossman, HB;Messing, E;Shen, Y
通讯作者：
Shen, Y
影响因子：
2
作者：
Miyake M;Goodison S;Giacoia EG;Rizwani W;Ross S;Rosser CJ
通讯作者：
Rosser CJ