DETERMINATION OF ARGINASE ACTIVITY IN MACROPHAGES - A MICROMETHOD
DETERMINATION OF ARGINASE ACTIVITY IN MACROPHAGES - A MICROMETHOD
复制标题
DOI：
10.1016/0022-1759(94)90027-2
复制
发表时间：
1994-09-14
影响因子：
2.2
通讯作者：
MODOLELL, M
中科院分区：
文献类型：
--
作者：
CORRALIZA, IM;CAMPO, ML;MODOLELL, M
我们提出对希姆克尿素测定方法进行修改，作为测量活化巨噬细胞中精氨酸酶的有价值的微方法。该方法具有以下优点：(a) 使用少量样品（每次测定约 25000 个巨噬细胞）； (b) 它不干扰活化巨噬细胞中也存在的其他相关代谢物，例如瓜氨酸或精氨酸； (c) 可以使用底物精氨酸的饱和浓度； (d)它比Schimke方法灵敏得多，可以检测0.02μmol量级的少量尿素。
We propose a modification of Schimke's method for urea determination as a valuable micromethod for measuring arginase in activated macrophages. The method exhibits the following advantages: (a) it uses small amounts of samples (approximately 25000 macrophages per assay); (b) it does not interfere with other related metabolites that are also present in the activated macrophage such as citrulline or arginine; (c) saturating concentrations of the substrate arginine can be used; and (d) it is much more sensitive than Schimke's method and can detect small amounts of urea, in the order of 0.02 mu mol.