Photobiomodulation preconditioning prevents cognitive impairment in a neonatal rat model of hypoxia-ischemia
Photobiomodulation preconditioning prevents cognitive impairment in a neonatal rat model of hypoxia-ischemia
复制标题
光生物调节预处理可预防新生大鼠缺氧缺血模型中的认知障碍
DOI：
10.1002/jbio.201800359
复制
发表时间：
2019
影响因子：
2.8
通讯作者：
Zhang Quanguang
中科院分区：
文献类型：
--
作者：
Yang Luodan;Dong Yan;Wu Chongyun;Li Yong;Guo Yichen;Yang Baocheng;Zong Xuemei;Hamblin Michael R.;Liu Timon C. -Y.;Zhang Quanguang
缺氧引起的新生儿缺氧缺血（HI）损伤是新生儿死亡和严重神经功能缺损的最常见原因。目前的工作评估了光生物调节 (PBM) 预处理的预防作用及其对新生大鼠 HI 模型中脑损伤的潜在作用机制。根据正常大鼠脑样本中 ATP 水平的最佳时间响应，在 HI 前 6 小时将 PBM 预处理 (PBM-P) 方案（808 nm CW 激光、1 cm2 光斑、100 mW/cm2、12 J/cm2）传送到头皮。 PBM-P 显着减轻 HI 后的认知障碍、大脑体积萎缩、神经元损失、树突和突触损伤。进一步的机制研究发现，PBM-P 可以恢复 HI 诱导的线粒体动力学并抑制线粒体断裂，随后强烈抑制细胞色素 c 的释放，并通过抑制 caspase 激活来预防神经元凋亡。我们的工作表明，PBM-P 可以通过维持线粒体动力学和抑制线粒体凋亡途径来减轻 HI 引起的脑损伤。
Neonatal hypoxia‐ischemia (HI) injury caused by oxygen deprivation is the most common cause of mortality and severe neurologic deficits in neonates. The present work evaluated the preventative effect of photobiomodulation (PBM) preconditioning, and its underlying mechanism of action on brain damage in an HI model in neonatal rats. According to the optimal time response of ATP levels in brain samples removed from normal rats, a PBM preconditioning (PBM‐P) regimen (808 nm CW laser, 1 cm2spot, 100 mW/cm2, 12 J/cm2) was delivered to the scalp 6 hours before HI. PBM‐P significantly attenuated cognitive impairment, volume shrinkage in the brain, neuron loss, dendritic and synaptic injury after HI. Further mechanistic investigation found that PBM‐P could restore HI‐induced mitochondrial dynamics and inhibit mitochondrial fragmentation, followed by a robust suppression of cytochrome c release, and prevention of neuronal apoptosis by inhibition of caspase activation. Our work suggests that PBM‐P can attenuate HI‐induced brain injury by maintaining mitochondrial dynamics and inhibiting the mitochondrial apoptotic pathway.