Phase-sensitive optical coherence tomography imaging of the tissue motion within the organ of Corti at a subnanometer scale: a preliminary study
Phase-sensitive optical coherence tomography imaging of the tissue motion within the organ of Corti at a subnanometer scale: a preliminary study
复制标题
DOI：
10.1117/1.3486543
复制
发表时间：
2010-09-01
影响因子：
3.5
通讯作者：
Nuttall, Alfred L.
中科院分区：
文献类型：
--
作者：
Wang, Ruikang K.;Nuttall, Alfred L.
听力损失会严重影响生活质量。然而，听力器官，即Corti器官（OC）如何响应声音的生物力学机制仍然难以捉摸，很大程度上是因为目前没有办法对OC的三维运动特征进行成像。我们提出了一种新的使用相敏光谱域光学相干断层扫描（PSOCT）在亚纳米尺度上表征OC内细胞区室的运动。psot系统工作在1310 nm，空间分辨率接近16 μ m，成像速度为47,000 a -lines/s。利用局部组织运动引起的光谱干涉图的相位变化来量化振动幅度。相位变化的傅里叶变换分析提高了系统对小于1nm的微小振动的灵敏度。我们证明，PSOCT系统是可行的成像有意义的振动细胞室在OC内具有前所未有的灵敏度低至类似于0.5埃(C) 2010年光电仪器工程师学会。(DOI: 10.1117/1.3486543)
Hearing loss can mean severe impairment to the quality of life. However, the biomechanical mechanisms of how the hearing organ, i.e., the organ of Corti (OC), responds to sound are still elusive, largely because there is currently no means available to image the 3-D motion characteristics of the OC. We present a novel use of the phase-sensitive spectral domain optical coherence tomography (PSOCT) to characterize the motion of cellular compartments within the OC at a subnanometer scale. The PSOCT system operates at 1310 nm with a spatial resolution of similar to 16 mu m and an imaging speed of 47,000 A-lines/s. The phase changes of the spectral interferograms induced by the localized tissue motion are used to quantify the vibration magnitude. Fourier transform analysis of the phase changes improves the system sensitivity to sense minute vibrations smaller than 1 nm. We demonstrate that the PSOCT system is feasible to image the meaningful vibration of cellular compartments within the OC with an unprecedented sensitivity down to similar to 0.5 angstrom (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3486543]