Wnt/β-catenin regulates an ancient signaling network during zebrafish scale development
Wnt/β-catenin regulates an ancient signaling network during zebrafish scale development
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DOI：
10.7554/elife.37001
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发表时间：
2018-07-17
期刊：
影响因子：
7.7
通讯作者：
Parichy, David M.
中科院分区：
文献类型：
--
作者：
Aman, Andrew J.;Fulbright, Alexis N.;Parichy, David M.
了解图案如何影响细胞行为以产生三维形态是发育生物学的中心目标。此外，比较这些调节机制之间的形态不同的组织允许严格的进化假说的测试。斑马鱼的皮肤被赋予了一层精确图案化的骨质鳞片。我们在鳞片发育和细胞信号传导活动的操纵过程中使用intoto实时成像来阐明鳞片图案化和形态发生的核心特征。这些分析表明，鳞片发育需要Wnt/β-连环蛋白、外胚层发育异常蛋白（Eda）和成纤维细胞生长因子（Fgf）信号传导的协同活性。该调节模块协调表皮内陷过程中Hedgehog（HH）依赖的集体细胞迁移，这是一种以前与皮肤附属器形态发生无关的细胞行为。我们的分析表明，作为一个听话的系统，以阐明发展模式和形态发生的机制，斑马鱼鳞片发展的效用，并提出了一个单一的，古老的起源在脊椎动物的皮肤附属器图案机制。
Understanding how patterning influences cell behaviors to generate three dimensional morphologies is a central goal of developmental biology. Additionally, comparing these regulatory mechanisms among morphologically diverse tissues allows for rigorous testing of evolutionary hypotheses. Zebrafish skin is endowed with a coat of precisely patterned bony scales. We use intoto live imaging during scale development and manipulations of cell signaling activity to elucidate core features of scale patterning and morphogenesis. These analyses show that scale development requires the concerted activity of Wnt/beta-catenin, Ectodysplasin (Eda) and Fibroblast growth factor (Fgf) signaling. This regulatory module coordinates Hedgehog (HH) dependent collective cell migration during epidermal invagination, a cell behavior not previously implicated in skin appendage morphogenesis. Our analyses demonstrate the utility of zebrafish scale development as a tractable system in which to elucidate mechanisms of developmental patterning and morphogenesis, and suggest a single, ancient origin of skin appendage patterning mechanisms in vertebrates.