Simultaneous time- and wavelength-resolved fluorescence spectroscopy for near real-time tissue diagnosis
Simultaneous time- and wavelength-resolved fluorescence spectroscopy for near real-time tissue diagnosis
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DOI：
10.1364/ol.33.000630
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发表时间：
2008-03-15
期刊：
影响因子：
3.6
通讯作者：
Marcu, Laura
中科院分区：
文献类型：
--
作者：
Sun, Yinghua;Liu, Rui;Marcu, Laura
展示了一种新型的基于光纤的同时时间和波长分辨荧光光谱法，用于快速诊断病变组织。通过将多个带通和二向色滤波器（405/40、460/50和550/50）与充当光学延迟的不同长度的光纤（1、10和19 m）相结合，该系统能够使用单个检测器和激发输入来实现时间分辨荧光光谱的近实时采集和表征。在选定波长下的多个荧光响应脉冲的记录可以在数百纳秒内完成，这提供了实时表征生物系统的能力。(C)2008年美国光学学会。
A novel fiber-optic-based method for simultaneous time- and wavelength-resolved fluorescence spectroscopy for the rapid diagnosis of diseased tissue is demonstrated. By combining multiple bandpass and dichroic filters (405/40, 460/50, and 550/50) with different lengths of optical fiber (1, 10, and 19 m) acting as an optical delay this system enables the near real-time acquisition and characterization of time-resolved fluorescence spectra using a single detector and excitation input. The recording of multiple fluorescence response pulses at selected wavelengths can be completed in hundreds of nanoseconds, which provides the capability of a real-time characterization of biological systems. (C) 2008 Optical Society of America.