The respiratory epithelium. VI. Histogenesis of lung tumors induced by benzo[a]pyrene-ferric oxide in the hamster.
The respiratory epithelium. VI. Histogenesis of lung tumors induced by benzo[a]pyrene-ferric oxide in the hamster.
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呼吸道上皮。
DOI：
10.1093/jnci/61.2.607
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
Benjamin F. Trump
中科院分区：
文献类型：
--
作者：
Peter J. Becci;Elizabeth M. McDowell;Benjamin F. Trump
用苯并[a]芘-Fe_2O_3诱发雌性叙利亚金黄地鼠肺肿瘤。肿瘤的特点是使用协调的形态学和组织化学技术，包括电子显微镜。肺癌根据其假定的起源细胞进行分类。大多数来源于粘液细胞和/或基底细胞，它们被分类为表皮样癌或表皮样癌和腺癌的组合。第二组的肿瘤（占癌总数的57%）呈现出广泛的表皮样和腺瘤成分。表皮样成分的特点是在分化良好的肿瘤细胞间桥和/或角化的存在。超微结构观察到发达的桥粒和许多张力细丝束。在诊断腺癌时，不再需要依赖于小管或大体腺体结构的存在作为诊断标准。细胞内和/或细胞外肺泡、发育良好的高尔基复合体和内质网的存在和/或粘液分泌的证据提供了更明确的标准。观察到一个由神经分泌细胞组成的肿瘤，其形态类似于人的支气管类癌。在光镜下可见均匀、小、多边形细胞巢，细胞核为圆形至卵圆形。肿瘤细胞胞浆内有致密的核心分泌颗粒，直径为1，100 - 2，200 A。观察到几个纤维肉瘤。肿瘤显示出非常细胞化的结构，由密集的卵圆形或梭形细胞组成。超微结构上，细胞类似成纤维细胞。本研究中获得的结果有力地支持了组织发生学分类，即，基于细胞起源的分类。
Lung tumors were induced in female Syrian golden hamsters by intratracheal instillation of benzo[a]pyrene-Fe2O3. The tumors were characterized with the use of coordinated morphologic and histochemical techniques including electron microscopy. The lung carcinomas were classified according to their presumed cell of origin. Most were derived from mucous cells and/or basal cells, and they were classified as either epidermoid carcinomas or as combined epidermoid and adenocarcinomas. The tumors in the second group (57% of the total number of carcinomas) presented a wide spectrum of epidermoid and adeno components. The epidermoid component was characterized in well-differentiated tumors by the presence of intercellular bridges and/or keratinization. Well-developed desmosomes and numerous bundles of tonofilaments were observed ultrastructurally. In diagnosing adenocarcinoma, one no longer needs to depend on the presence of tubules or gross glandular structures as criteria for diagnosis. The presence of intracellular and/or extracellular alveoli, well-developed Golgi complex, and endoplasmic reticulum and/or evidence of mucous secretion provide more definitive criteria. A tumor composed of neurosecretory cells that morphologically resembled a bronchial carcinoid of man was observed. Nests of uniform, small, polygonal cells with round-to-oval nuclei were seen at the light microscopic level. Dense-core secretory granules 1,100-2,200 A were present in the cytoplasm of the tumor cells. Several fibrosarcomas were observed. The tumors showed a very cellular structure, composed of either densely packed ovoid or spindle-shaped cells. Ultrastructurally, the cells resembled fibroblasts. The results obtained in this study give strong support for a histogenetic classification, i.e., a classification based on the cell of origin.