The velvet-activated putative C6 transcription factor VadZ regulates development and sterigmatocystin production in Aspergillus nidulans
The velvet-activated putative C6 transcription factor VadZ regulates development and sterigmatocystin production in Aspergillus nidulans
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DOI：
10.1016/j.funbio.2022.05.001
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发表时间：
2022-06-01
期刊：
影响因子：
2.5
通讯作者：
Yu, Jae-Hyuk
中科院分区：
文献类型：
--
作者：
Zhao, Yanxia;Lee, Mi-Kyung;Yu, Jae-Hyuk
NF-κ B型VosAeVelB天鹅绒复合物作为一个全球性的调节剂，管理真菌的发育和代谢。VosA-VelB激活的发育（VAD）基因之一vadZ被预测编码一个557个氨基酸的蛋白质，该蛋白质含有构巢曲霉中高度保守的GAL 4型Zn（II）（2）Cys（6）（或C-6锌）双核簇DNA结合结构域。在这份报告中，我们描述了vadZ基因在控制发育和杂色曲霉素（ST）生产的功能。nidulans。为了验证VosA-VelB介导的vadZ激活，我们检查了在营养、无性和有性发育阶段野生型（WT）、Delta vosA和Delta velB突变株中vadZ的相对mRNA水平。在无性发育开始时，vosA的缺失导致vadZ mRNA水平降低66.2倍，而Delta velB导致vadZ mRNA水平降低3.6倍。vadZ的缺失导致菌落生长受到明显限制，同时无性发育减少，但增加了称为闭囊藻的有性子实体的形成。此外，无效vadZ导致整个生命周期中两个关键性发育激活因子esdC和nsdD的mRNA水平升高。此外，Delta vadZ突变体显示ST的产量升高，ST生物合成基因的mRNA水平增强。综上所述，推测的C-6转录因子VadZ促进A.
The NF-kappa B-type VosAeVelB velvet complex acts as a global regulator governing development and metabolism in fungi. One of the VosA-VelB-activated developmental (VAD) genes called vadZ is predicted to encode a 557-amino acid protein containing a highly conserved GAL4-type Zn(II)(2)Cys(6) (or C-6 zinc) binuclear cluster DNA-binding domain in Aspergillus nidulans. In this report, we characterize the function of the vadZ gene in controlling development and sterigmatocystin (ST) production in A. nidulans. To verify VosA-VelB mediated activation of vadZ, we checked relative mRNA levels of vadZ in wild-type (WT), Delta vosA, and Delta velB mutant strains during vegetative, asexual, and sexual development phases. At the beginning of asexual development, the absence of vosA led to a 66.2-fold lowered vadZ mRNA levels, whereas Delta velB resulted in a 3.6-fold decrease in vadZ mRNA levels. The deletion of vadZ resulted in significantly restricted colony growth coupled with reduced asexual development, but increased formation of sexual fruiting bodies called cleistothecia. In addition, nullifying vadZ caused elevated mRNA levels of the two key sexual developmental activators esdC and nsdD throughout the lifecycle. Moreover, the Delta vadZ mutant showed elevated production of ST and enhanced mRNA levels of ST biosynthetic genes. In summary, the putative C-6 transcription factor VadZ promotes asexual development and suppresses the sexual development and the ST production in A.