On-virus construction of polyvalent glycan ligands for cell-surface receptors
On-virus construction of polyvalent glycan ligands for cell-surface receptors
复制标题
DOI：
10.1021/ja077801n
复制
发表时间：
2008-04-09
影响因子：
15
通讯作者：
Finn, M. G.
中科院分区：
文献类型：
--
作者：
Kaltgrad, Eiton;O'Reilly, Mary K.;Finn, M. G.
排列在病毒颗粒外部的聚糖被用作糖基转移酶反应的底物，以从病毒表面构建二聚体和三聚体。所得颗粒表现出与同源受体珠和细胞的紧密和特异性缔合，在一个实例中以多价结合的特征性方式击败顺式细胞表面相互作用。结合病毒提供结构明确的附着点的能力，该方法提供了一种方便和强大的方式来制备成簇受体的复杂碳水化合物配体。
Glycans arrayed on the exterior of virus particles were used as substrates for glycosyltransferase reactions to build di- and trisaccharides from the virus surface. The resulting particles exhibited tight and specific associations with cognate receptors beads and cells, in one example defeating in cis cell-surface interactions in a manner characteristic of polyvalent binding. Combined with the ability of viruses to provide structurally well-defined attachment points, the methodology provides a convenient and powerful way to prepare complex carbohydrate ligands for clustered receptors.