Hepatitis c virus quasispecies in HIV-infected women: Role of injecting drug use and highly active antiretroviral therapy (HAART)
Hepatitis c virus quasispecies in HIV-infected women: Role of injecting drug use and highly active antiretroviral therapy (HAART)
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DOI：
10.1002/hep.21715
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发表时间：
2007-08-01
期刊：
影响因子：
13.5
通讯作者：
Kovacs, Andrea
中科院分区：
文献类型：
--
作者：
Laskus, Tomasz;Wilkinson, Jeffrey;Kovacs, Andrea
尽管丙型肝炎病毒和艾滋病毒混合感染的频率很高，但对艾滋病毒阳性患者中的丙型肝炎病毒准种知之甚少。目前的分析包括236名参加妇女机构间艾滋病毒研究(WIHS)的艾滋病毒+/抗-丙型肝炎+妇女。用单链构象多态(SSCP)方法分析第二外膜基因高变区I。用多因素模型分析丙型肝炎病毒准种与临床和人口学特征的关系。年龄超过40岁和高丙型肝炎病毒RNA载量是唯一与准种复杂性显著相关的因素，评估为SSCP条带数量。如稳定的SSCP手纹所反映的那样，高的艾滋病毒和丙型肝炎病毒血浆载量与准种随时间的稳定性有关。然而，积极注射毒品的女性经历准种变化的可能性是不注射的女性的3倍。与CD4计数或高效抗逆转录病毒治疗(HAART)相关的丙型肝炎病毒准种动态没有受到影响。结论：在人类免疫缺陷病毒/丙型肝炎病毒混合感染者中，丙型肝炎病毒准种的复杂性和动态与HIV和丙型肝炎病毒血浆载量的相关性比与CD4+细胞计数的相关性更密切。活跃的药物使用与准种变化有关，可能是由于反复重复感染新的丙型肝炎病毒株所致。在规划对合并感染者的丙型肝炎病毒的治疗和预防策略时，需要考虑这一点。
Despite the high frequency of HCV and HIV coinfection, little is known about HCV quasispecies in HIV-positive patients. The current analysis included 236 HIV+/anti-HCV+ women enrolled in the Women's Interagency HIV Study (WIHS). Hypervariable region I of the second envelope gene was analyzed by single-strand conformation polymorphism (SSCP). The relationship between the HCV quasispecies and clinical and demographic features were analyzed in multivariate models. Age over 40 years and high HCV RNA load were the only factors significantly associated with quasispecies complexity, assessed as the number of SSCP bands. High HIV and HCV plasma loads were associated with quasispecies stability over time, as reflected by stable SSCP hand patterns. However, women who were actively injecting drugs were 3 times more likely to experience quasispecies changes than their noninjecting counterparts. No affect on HCV quasispecies dynamics was noted in relation to CD4 count or highly active antiretroviral therapy (HAART). Conclusion: among HIV/HCV coinfected patients, HCV quasispecies complexity and dynamics correlate more closely with HIV and HCV plasma loads than with CD4+ cell counts. Active drug use is associated with quasispecies changes probably due to repeated superinfections with new HCV strains. This needs to be considered when planning treatment and prevention strategies for HCV in coinfected individuals.