Identification of RNA binding specificity for the TET-family proteins.
Identification of RNA binding specificity for the TET-family proteins.
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DOI：
10.1093/nass/nrn108
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发表时间：
2008-01-01
期刊：
影响因子：
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通讯作者：
Oyoshi, Takanori
中科院分区：
文献类型：
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作者：
Takahama, Kentaro;Kino, Katsuhito;Oyoshi, Takanori
TET家族蛋白（TAF 15、EWS和TLS）是参与多水平细胞功能的RNA结合蛋白。这些蛋白质的RNA结合结构域被认为是细胞功能的重要区域。但对TET家族蛋白的RNA结合特异性知之甚少。为了研究TET家族蛋白的RNA结合特性，我们使用重组Flag-taged TLS和富含鸟嘌呤的RNA进行电泳迁移率变动分析。发现TLS在KCl存在下与人端粒RNA结合，但在LiCl存在下不结合。
The TET-family proteins (TAF15, EWS and TLS) are the RNA binding proteins involved in multiple levels of cellular functions. The RNA binding domain of those proteins is known as the important region for cellular functions. But little is known about the RNA binding specificity of TET-family proteins. In order to investigate the RNA binding properties of the TET-family proteins, we performed electrophoretic mobility shift assay using recombinant Flag-tagged TLS and guanine-rich and RNAs. It was found that TLS binds to human telomeric RNA in the presence of KCl, but not in the presence of LiCl.