Isolation and characterization of new microsatellite markers from the Japanese scallop (Patinopecten yessoensis)
Isolation and characterization of new microsatellite markers from the Japanese scallop (Patinopecten yessoensis)
复制标题
DOI：
10.1007/s10592-009-9900-6
复制
发表时间：
2010-06-01
影响因子：
2.2
通讯作者：
Sun, Xiao-Wen
中科院分区：
文献类型：
--
作者：
Chen, Meng;Chang, Ya-Qing;Sun, Xiao-Wen
采用两次杂交的方法从虾夷扇贝群体中分离到20个多态性微卫星位点。利用这些微卫星标记分析了辽宁省獐子岛野生样品的遗传多样性。计算观察杂合度（H（o））、期望杂合度（H（e））、有效等位基因数（N（e））和多态信息含量（PIC）。用固定指数（Fis）检测杂合子的缺失或过量。Hardy-Weinberg平衡用可能性（P（HWE））检验。结果表明，平均等位基因数为6.0。参数H（o）、H（e）、N（e）和PIC的平均值分别为0.7921、0.7076、4.4347和0.6684。20个微卫星标记中只有4个标记（FJ 262370、FJ 262381、FJ 262384和FJ 262400）显示杂合子缺失。P（HWE）值表明野生种群偏离了Hardy-Weinberg平衡。这些新分离的分子标记增加了日本扇贝的可用分子资源。
Twenty polymorphic microsatellite loci were isolated from the Japanese scallop (Patinopecten yessoensis) using a method of hybridizing twice. The genetic diversity was analyzed by these microsatellite markers in field specimens from Zhangzidao Island in Liaoning Province, China. Observed heterozygosity (H (o)), expected heterozygosity (H (e)), number of effective alleles (N (e)), and polymorphism information contents (PIC) were calculated. The heterozygote deficiency or excess was detected by the fixation index (Fis). The Hardy-Weinberg equilibrium was tested by possibilities (P (HWE)) using chi-square test. Results indicate that the average of allele numbers was 6.0. The mean values of the parameters H (o), H (e), N (e), and PIC were 0.7921, 0.7076, 4.4347, and 0.6684, respectively. Only 4 of 20 microsatellite markers showed heterozygote deficiency including loci FJ262370, FJ262381, FJ262384, and FJ262400. The P (HWE) value indicates the wild population deviated somewhat from the Hardy-Weinberg equilibrium. These newly isolated markers increase the available molecular resources of the Japanese scallop.