Generation, Endothelialization, and Microsurgical Suture Anastomosis of Strong 1-mm-Diameter Collagen Tubes
Generation, Endothelialization, and Microsurgical Suture Anastomosis of Strong 1-mm-Diameter Collagen Tubes
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DOI：
10.1089/ten.tea.2016.0339
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发表时间：
2017-04-01
影响因子：
4.1
通讯作者：
Tien, Joe
中科院分区：
文献类型：
--
作者：
Li, Xuanyue;Xu, Jing;Tien, Joe
基于重组细胞外基质的组织工程血管移植物一直受到机械强度较弱的困扰，使其无法处理或吻合到天然血管。在这项研究中，我们设计了一种方法，通过脱水天然大鼠尾部I型胶原管并用20 mM京尼平对其进行交联，制备出直径为1 mm的致密、可缝合的胶原管结构，用于潜在的显微外科应用。内径1 mm的交联型致密胶原管的极限拉伸强度为342+/-15gF，破裂压力为1313+/-156 mm Hg，与大鼠股动脉的强度相当，并支持内皮细胞的黏附和生长。直径0.5 mm的管端对端吻合移植动脉显示出82+/-21gF的吻合强度，足以满足外科应用。在大鼠股循环内植入无细胞管间置式移植物，20min后吻合稳定，血流量稳定。种植致密的胶原管是静脉移植物的一种有前途的替代方案，可用于再植手术中短动脉残端之间的桥接。
Tissue-engineered vascular grafts that are based on reconstituted extracellular matrices have been plagued by weak mechanical strength that prevents handling or anastomosis to native vessels. In this study, we devise a method for making dense, suturable collagen tubular constructs of diameter 1mm for potential microsurgical applications, by dehydrating tubes of native rat tail type I collagen and crosslinking them with 20mM genipin. Crosslinked dense collagen tubes with 1mm inner diameter yielded ultimate tensile strength of 342 +/- 15gF and burst pressure of 1313 +/- 156mm Hg, comparable to the strength of a rat femoral artery, and supported endothelial cell adhesion and growth. End-to-end anastomosis of 0.5-mm-diameter tubes to explanted arteries displayed anastomotic strength of 82 +/- 21gF, which is sufficient for surgical applications. In vivo implantation of cell-free tubes as interpositional grafts in the rat femoral circulation yielded stable anastomosis with blood flow for 20min. Seeded dense collagen tubes represent a promising alternative to venous graft that can potentially be used to bridge between short artery stubs in replantation surgeries.