Chicken anaemia virus enhances and prolongs subsequent avian influenza (H9N2) and infectious bronchitis viral infections
Chicken anaemia virus enhances and prolongs subsequent avian influenza (H9N2) and infectious bronchitis viral infections
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DOI：
10.1016/j.vetmic.2019.01.024
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发表时间：
2019-03-01
影响因子：
3.3
通讯作者：
Naguib, Mahmoud M.
中科院分区：
文献类型：
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作者：
Erfan, Ahmed M.;Selim, Abdullah A.;Naguib, Mahmoud M.
由于继发感染的易感性增加，免疫抑制性病毒性疾病在家禽业中具有重大的经济重要性。鸡贫血病毒(CAV)是鸡的主要免疫抑制疾病之一。此外，H9N2亚型低致病性禽流感（LPAI）和传染性支气管炎（IB）病毒是全球最常报告的家禽呼吸道病毒性疾病。在本研究中，使用无特定病原体的鸡来了解 CAV 对 LPAI-H9N2 或 1B 病毒继发感染的影响。评估了临床结果、病毒脱落动态和细胞因子水平。结果表明，先前感染过 CAV 的鸡在口咽拭子、气管和肾脏中产生的 LPAI-H9N2 或 IB 病毒滴度要高得多（P < 0.05）。此外，CAV 的免疫作用引发了 LPAI-H9N2 和 IB 病毒感染的临床症状。此外，结果表明，CAV 预感染与 IL-6 和 IFN γ 水平升高直接相关。这些发现强调了 CAV 预感染对鸡 LPAI-H9N2 或 IB 感染的重要性，并表明 CAV 的共循环可能有助于 LPAI H9N2 和 IB 病毒的传播和进化。
Immunosuppressive viral diseases have a great economic importance in the poultry industry due to the increased susceptibility to secondary infections. Chicken anaemia virus (CAV) is one of the major immunosuppressive diseases in chickens. In addition, low pathogenic avian influenza (LPAI) of subtype H9N2 and infectious bronchitis (IB) viruses are among the most frequently reported respiratory viral diseases in poultry worldwide. In the present study, specific pathogen free chickens were used to understand the impact of CAV on secondary infection with LPAI-H9N2 or 1B viruses. Clinical outcomes, viral shedding dynamics, and cytokine levels wereassessed. The results exhibit that chickens previously infected with CAV produceconsiderablyhigher titresof LPAI-H9N2 or IB viruses in the oropharyngeal swabs (P < 0.05), tracheas and kidneys. In addition, the immunologic effect of CAV provokedthe development of clinical signs of LPAI-H9N2 and IB virus infections. Moreover, results suggested that pre-infection with CAV directly correlated with elevated levels of IL-6 and IFN gamma. These findings underline the importance of CAV pre-infection on LPAI-H9N2 or IB infection in chickens, and indicate that co-circulation of CAV can contribute to the spread and evolution of LPAI H9N2 and IB viruses.