Arboviral Survey of Mosquitoes in Two Northern Italian Regions in 2007 and 2008
Arboviral Survey of Mosquitoes in Two Northern Italian Regions in 2007 and 2008
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DOI：
10.1089/vbz.2009.0176
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发表时间：
2010-11-01
影响因子：
2.1
通讯作者：
Dottori, Michele
中科院分区：
文献类型：
--
作者：
Calzolari, Mattia;Bonilauri, Paolo;Dottori, Michele
最近，意大利-特别是埃米利亚-罗马涅地区-连续爆发了由虫媒病毒基孔肯雅病毒和西尼罗河病毒引起的人类疾病。这两次爆发是由不同种类的蚊子传播的，没有关联，但指出该地区缺乏虫媒病毒监测计划。从2007年开始，在埃米利亚-罗马涅地区启动了昆虫学监测，2008年，该方案得到了改进，并在隆巴迪亚地区得到推广。使用CO2诱捕器，收集了65，292只蚊子;按收集日期、地点和物种合并;手动浸软;并通过逆转录（RT）-聚合酶链反应检测甲病毒、正布尼亚病毒和黄病毒的存在。对扩增子进行测序，并通过GenBank中的基本局部比对搜索工具搜索用于鉴定病毒RNA。这些测定的结果显示：（1）两个库蚊池中存在西尼罗病毒，（2）两种正布尼亚病毒的RNA存在，在一个Ochlerotatus caspius蚊子池中存在Tahyna病毒，在一个Anopheles maculipennis蚊子池中存在Batai病毒，和（3）在Oc.这些扩增子的序列与仅在蚊子中检测到并且没有公认的脊椎动物宿主的黄病毒（伊蚊黄病毒、库蚊黄病毒和卡米蒂河病毒）最密切相关。
Recently, Italy-particularly the Emilia-Romagna region-was the location of consecutive outbreaks of human diseases caused by the arboviruses chikungunya virus and West Nile virus. The two outbreaks, spread by different species of mosquitoes, were not related, but pointed out the lack of an arboviral surveillance program in this region. Beginning in 2007 entomological surveillance was initiated in the Emilia-Romagna region, and in 2008 the program was improved and extended at Lombardia region. Using CO2-baited traps, 65,292 mosquitoes were collected; pooled by date of collection, location, and species; macerated manually; and tested by reverse transcription (RT)-polymerase chain reaction for the presence of alphaviruses, orthobunyaviruses, and flaviviruses. Amplicons were sequenced and employed for identification of viral RNA by basic local alignment search tool search in GenBank. Results of these assays showed (1) the presence of West Nile virus in two pools of Culex pipiens mosquitoes, (2) the presence of RNA of two orthobunyaviruses, Tahyna virus in a pool of Ochlerotatus caspius mosquitoes and Batai virus in a pool of Anopheles maculipennis mosquitoes, and (3) the presence of flavivirus RNAs in pools of Oc. caspius, Aedes albopictus, and Aedes vexans mosquitoes; the sequences of these amplicons were most closely related to flaviviruses that have been detected only in mosquitoes and had no recognized vertebrate host (Aedes flavivirus, Culex flavivirus, and Kamiti River virus).