Transcriptome analysis of bagging-treated red Chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation.
Transcriptome analysis of bagging-treated red Chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation.
复制标题
套袋处理的红沙梨皮的转录组分析揭示了花青素积累中的光响应途径功能
DOI：
10.1038/s41598-017-00069-z
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发表时间：
2017-03-03
影响因子：
4.6
通讯作者：
Teng Y
中科院分区：
文献类型：
--
作者：
Bai S;Sun Y;Qian M;Yang F;Ni J;Tao R;Li L;Shu Q;Zhang D;Teng Y
套袋是促进果实着色的有效方法。本研究以套袋处理的红砂梨果皮为材料，进行转录组分析。通过加权基因共表达网络分析，总共分析了8，870个差异表达基因，并鉴定了早期、中期和晚期光响应基因。注释分析揭示了几个途径参与不同的反应阶段。LONG HYPOCOTLY 5、RAD-DAS和aCONSTANS-like转录因子在早期光响应基因中的存在表明光，特别是蓝光，在袋去除后发生的生物学变化中起关键作用。其他光响应转录因子也被确定从三个光响应阶段。此外，花色素苷生物合成基因的光响应模式在生物合成步骤之间存在差异。虽然酵母单杂交试验表明，大部分结构基因都受PpMYB 10的调控，但它们不同的表达模式表明，除了PpMYB 10外，还有其他光响应转录因子参与了花青素合成的调控。总之，我们的转录组分析提供了在光响应过程中操作的转录调控网络的知识，这导致花青素积累和其他显着的生理变化，在红砂梨果皮后，袋去除。
Bagging is an efficient method to improve fruit colour development. This work reported a transcriptome analysis using bagging-treated red Chinese sand pear peels. In total, 8,870 differentially expressed genes were further analysed by a weighted gene co-expression network analysis and early-, middle- and late light-responsive genes were identified. An annotation analysis revealed several pathways involved in the different responsive stages. The presence ofLONG HYPOCOTLY 5,CRY-DASHand aCONSTANS-liketranscription factors among the early light-responsive genes indicated the pivotal role of light, especially blue light, in the biological changes that occurred after bag removal. Other light-responsive transcription factors were also identified from the three light-responsive stages. In addition, the light-responsive pattern of anthocyanin biosynthetic genes differed among the biosynthetic steps. Although yeast-one hybrid assay showed that most of the structural genes were regulated by PpMYB10, their different temporal expressive pattern suggested that besides PpMYB10, other light-responsive transcriptional factors were also involved in the regulation of anthocyanin biosynthesis. In summary, our transcriptome analysis provides knowledge of the transcriptional regulatory network operating during light responses, which results in anthocyanin accumulation and other significant physiological changes in red Chinese sand pear peels after bag removal.