Physiology of IgD. IV. Enhancement of antibody production in mice bearing IgD-secreting plasmacytomas.
Physiology of IgD. IV. Enhancement of antibody production in mice bearing IgD-secreting plasmacytomas.
复制标题
Igd的生理学。 iv。增强含有IgD分泌浆细胞瘤的小鼠中抗体产生的增强。
DOI：
10.1084/jem.159.1.103
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发表时间：
1984-01-01
影响因子：
15.3
通讯作者：
Thorbecke, G J
中科院分区：
文献类型：
--
作者：
Xue, B;Coico, R;Wallace, D;Siskind, G W;Pernis, B;Thorbecke, G J
在皮下移植了两种已知的分泌igd的BALB/c浆细胞瘤TEPC-1017和TEPC-1033的BALB/c小鼠中，研究了对三硝基苯化血青素（TNP-KLH）、菲科尔（TNP-Ficoll）和流产布鲁氏菌（TNP-BA）的免疫应答。静脉注射TNP-KLH后，脾脏原发性和继发性19S和7S脾斑块形成细胞（PFC）反应增强了3 - 5倍。对TNP-Ficoll的主要反应是对照小鼠的1.5-2倍（特别是7S PFC反应）。TEPC-1017增强了对TNP-BA的初级应答，而TEPC-1033抑制了对TNP-BA的次级应答，而两种肿瘤对TNP-BA的次级应答均增强了3至7倍。腹腔注射携带TEPC-1017或TEPC-1033的小鼠的腹水，或从这些腹水中分离出的IgD，与肿瘤本身一样，引起对tnf - klh的初级反应的类似增强，特别是在抗原注射前大约1周注射时。含igd的腹水对胸腺（nu/nu） BALB/c小鼠对TNP-KLH的反应无影响。这些发现提示存在igd应答性免疫调节性T细胞。
Immune responses to trinitrophenylated hemocyanin (TNP-KLH), Ficoll (TNP-Ficoll), and Brucella abortus (TNP-BA) were examined in BALB/c mice bearing subcutaneous transplants of TEPC-1017 and TEPC-1033, the two known IgD-secreting BALB/c plasmacytomas. Both primary and secondary 19S and 7S splenic plaque-forming cell (PFC) responses in spleen to intravenously injected TNP-KLH were enhanced three to fivefold. Primary responses to TNP-Ficoll were 1.5-2 times higher than in control mice (particularly the 7S PFC response). Primary responses to TNP-BA were enhanced by TEPC-1017 but suppressed by TEPC-1033, while secondary responses to TNP-BA were enhanced three to sevenfold by both tumors. Intraperitoneal injections of ascites fluid from mice bearing TEPC-1017 or TEPC-1033, or of IgD isolated from such ascites fluid, caused a similar enhancement of the primary response to TNP-KLH, as did the tumor itself, particularly when injected approximately 1 wk before antigen injection. IgD-containing ascites fluid had no effect on the response of athymic (nu/nu) BALB/c mice to TNP-KLH. These findings suggest the existence of an IgD-responsive immunoregulatory T cell.