The Chinese prescription lianhuaqingwen capsule exerts anti-influenza activity through the inhibition of viral propagation and impacts immune function.
The Chinese prescription lianhuaqingwen capsule exerts anti-influenza activity through the inhibition of viral propagation and impacts immune function.
复制标题
DOI：
10.1186/s12906-017-1585-7
复制
发表时间：
2017-02-24
影响因子：
--
通讯作者：
Zhang F
中科院分区：
文献类型：
--
作者：
Ding Y;Zeng L;Li R;Chen Q;Zhou B;Chen Q;Cheng PL;Yutao W;Zheng J;Yang Z;Zhang F
连花清瘟胶囊是一种治疗呼吸道感染性疾病的中药方剂。本研究的目的是确定LH-C的抗病毒活性及其对病毒感染的免疫调节作用。采用MTT法和空斑减数法测定LH-C的体外细胞毒活性和抗病毒活性。使用单循环病毒生长条件下的时间进程研究来确定病毒复制的哪个阶段被阻断。LH-C对病毒核蛋白的核输出的影响使用间接免疫荧光测定法进行检查。采用Western blotting和real-time RT-PCR检测LH-C对不同信号转导事件和细胞因子/趋化因子表达的调节作用。BALB/c小鼠接种病毒后，连续5天灌胃不同浓度的LH-C。测定小鼠体重、病毒滴度、肺组织病理学改变，并采用实时荧光定量PCR检测小鼠体内炎性细胞因子水平。LH-C对多种流感病毒株的体外增殖均有抑制作用，其半数抑制浓度（IC 50）为0.35 ~ 2 mg/mL。LH-C阻断病毒感染的早期阶段（0-2 h），它还以剂量依赖性方式抑制病毒诱导的NF-κ B活化和减轻病毒诱导的IL-6、IL-8、TNF-α、IP-10和MCP-1的基因表达。LH-C处理有效地损害了病毒RNP的核输出。在LH-C给药组中观察到小鼠肺中病毒滴度降低。在感染的早期阶段，炎性细胞因子的水平也降低。LH-C作为一种中药方剂，对包括新出现的H7N9在内的一系列流感病毒具有广谱作用，特别是调节病毒感染的免疫反应。因此，LH-C可能是治疗流感病毒感染的一种有前途的选择。本文的在线版本（doi：10.1186/s12906-017-1585-7）包含补充材料，可供授权用户使用。
Lianhuaqingwen Capsule (LH-C) is a traditional Chinese medicine (TCM) formula used to treat respiratory tract infectious diseases in Chinese. The aim of this study was to determine the antiviral activity of LH-C and its immunomodulatory effects on viral infection. The in vitro cytotoxicity and antiviral activity of LH-C was determined by MTT and Plaque reduction assays. Time course study under single-cycle virus growth conditions were used to determine which stage of viral replication was blocked. The effect of LH-C on the nuclear export of the viral nucleoprotein was examined using an indirect immunofluorescence assay. The regulation to different signaling transduction events and cytokine/chemokine expression of LH-C was evaluated using Western blotting and real-time RT-PCR. After virus inoculation, BALB/c mice were administered with LH-C of different concentrations for 5 days. Body-weight, viral titers and lung pathology of the mice were measured, the level of inflammatory cytokines were also examined using real-time RT-PCR. LH-C inhibited the proliferation of influenza viruses of various strain in vitro, with the 50% inhibitory concentration (IC50) ranging from 0.35 to 2 mg/mL. LH-C blocked the early stages (0–2 h) of virus infection, it also suppressed virus-induced NF-kB activation and alleviated virus-induced gene expression of IL-6, IL-8, TNF-a, IP-10, and MCP-1 in a dose-dependent manner. LH-C treatment efficiently impaired the nuclear export of the viral RNP. A decrease of the viral titers in the lungs of mice were observed in groups administered with LH-C. The level of inflammatory cytokines were also decreased in the early stages of infection. LH-C, as a TCM prescription, exerts broad-spectrum effects on a series of influenza viruses, including the newly emerged H7N9, and particularly regulates the immune response of virus infection. Thus, LH-C might be a promising option for treating influenza virus infection. The online version of this article (doi:10.1186/s12906-017-1585-7) contains supplementary material, which is available to authorized users.