CORONAVIRUS-SD AND CORONAVIRUS-SK SHARE EXTENSIVE NUCLEOTIDE HOMOLOGY WITH MURINE CORONAVIRUS MHV-A59, MORE THAN THAT SHARED BETWEEN HUMAN AND MURINE CORONAVIRUSES
CORONAVIRUS-SD AND CORONAVIRUS-SK SHARE EXTENSIVE NUCLEOTIDE HOMOLOGY WITH MURINE CORONAVIRUS MHV-A59, MORE THAN THAT SHARED BETWEEN HUMAN AND MURINE CORONAVIRUSES
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DOI：
10.1016/s0042-6822(83)80022-1
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发表时间：
1983-01-01
期刊：
影响因子：
3.7
通讯作者：
WEISS, SR
中科院分区：
文献类型：
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作者：
WEISS, SR
使用代表小鼠肝炎病毒（MHV）A59株（MHV-A59）基因组的c[互补]DNA探针来测量鼠和人冠状病毒以及SD和SK冠状病毒之间的核苷酸序列同源性。由于SD和SK是通过将多发性硬化组织接种到小鼠或培养的小鼠细胞中分离的，因此确定它们与其他小鼠和人类冠状病毒分离株的关系非常重要。显然，SD和SK共享几乎完全的核苷酸同源性（约90%），并产生与MHV-A59相同大小的亚基因组RNA。检测的人冠状病毒（HCV）菌株与MHV-A59的同源性较低。免疫学无关的HCV-229 E显示与MHV-A59没有核苷酸同源性。免疫交叉反应的HCV-OC 43与MHV-A59经印迹杂交显示核苷酸同源性，但在溶液中杂交和经S1核酸酶消化测定时不显示核苷酸同源性。
A c[complementary]DNA probe representing the genome of mouse hepatitis virus (MHV) strain A59 (MHV-A59) was used to measure nucleotide sequence homologies among murine and human coronaviruses and the SD and SK coronaviruses. Since SD and SK were isolated by inoculation of multiple sclerosis tissue into mice or cultured mouse cells, it is important to determine their relationships to other murine and human coronavirus isolates. Evidently, SD and SK share almost complete nucleotide homology (.apprx. 90%) with MHV-A59 and generate subgenomic RNA of the same sizes as MHV-A59. The human coronavirus (HCV) strains tested show less homology with MHV-A59. The immunologically unrelated HCV-229E shows no nucleotide homology with MHV-A59. The immunologically cross-reactive HCV-OC43 shows nucleotide homology with MHV-A59 by blot hybridization but not when hybridized in solution and assayed by S1 nuclease digestion.