Alternatives in Donkey semen cryopreservation: Mare vs. Jenny Colostrum
Alternatives in Donkey semen cryopreservation: Mare vs. Jenny Colostrum
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DOI：
10.1111/rda.13516
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发表时间：
2019-10-01
影响因子：
1.7
通讯作者：
Gil, Lydia
中科院分区：
文献类型：
--
作者：
Alvarez, Cristina;Gonzalez, Noelia;Gil, Lydia
这项研究的目的是测试和比较用于冷冻驴精液的稀释剂中的两种新成分：马初乳和珍妮初乳。初乳是在小马驹出生后立即从四匹母马和四只小母马身上获得的。精液是从五头有生育能力的驴身上收集的。将精子样本混合、稀释和冷冻保存在三种不同的实验稀释剂组中：乳糖添加蛋黄稀释剂(20%)作为对照组，乳糖添加珍妮初乳稀释剂(20%)和乳糖添加母初乳稀释剂(20%)。解冻后用计算机辅助分析精子活力，SYBR-14和碘化丙啶(PI)法检测精子存活率，HOS法检测精子膜功能，异硫氰酸酯-花生凝集素(FITC-PNA)和PI检测精子顶体完整性。结果表明，解冻后乳糖-珍妮初乳稀释液的各项指标--总动力、存活率、HOS试验、VCL、VSL、VAP、LIN、STR和WOB均显著高于马初乳和蛋黄稀释液(P<0.05)。综上所述，将含有珍妮初乳的稀释液用于驴精液冷冻保存，提高了驴精液冷冻-解冻后的质量。
The aim of this study was to test and compare two new components in extenders for freezing donkey semen: mare colostrum and jenny colostrum. Colostrum was obtained from four mares and four jennies right after the foal's birth. Ejaculates were collected from five fertile donkeys. Sperm samples were pooled, diluted and cryopreserved in three different experimental extender groups: lactose supplemented with egg yolk extender (20%) as the control group, lactose supplemented with jenny colostrum extender (20%), and lactose supplemented with mare colostrum extender (20%). After thawing, we evaluated the sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodide (PI), membrane functional by HOS test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI. The results demonstrated that lactose-jenny colostrum extender displayed significantly higher values (p .05) in nearly all parameters evaluated - Total Motility, Viability, HOS test, VCL, VSL, VAP, LIN, STR and WOB -, compared with mare colostrum and egg yolk extenders after thawing. In conclusion, the extender containing jenny colostrum used for donkey semen cryopreservation improved the donkey sperm quality after the freezing-thawing process.