Assessment of red blood cell deformability in type 2 diabetes mellitus and diabetic retinopathy by dual optical tweezers stretching technique.
Assessment of red blood cell deformability in type 2 diabetes mellitus and diabetic retinopathy by dual optical tweezers stretching technique.
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DOI：
10.1038/srep15873
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发表时间：
2016-03-15
影响因子：
4.6
通讯作者：
Pavesio C
中科院分区：
文献类型：
--
作者：
Agrawal R;Smart T;Nobre-Cardoso J;Richards C;Bhatnagar R;Tufail A;Shima D;H Jones P;Pavesio C
使用双光镊拉伸技术进行了一项试点横断面研究，以研究红细胞 (RBC) 变形性在患有和患有糖尿病视网膜病变 (DR) 的 2 型糖尿病 (T2DM) 中的作用。双光镊是通过分离和重组单个 Nd:YAG 激光束制成的。红细胞被直接捕获（即没有微珠手柄）在双光镊中，观察到它们采用“侧向”方向。通过数字视频显微镜测量拉伸后红细胞的初始和最终长度，并计算变形指数（DI）。分析了 8 名健康对照、5 名 T2DM 和 7 名 DR 患者的血液，平均年龄分别为 52.4 岁、51.6 岁和 52 岁。对照组红细胞的初始平均长度为 8.45±0.25μm，DM 红细胞为 8.68±0.49μm，DR 红细胞为 8.82±0.32μm（p<0.001）。对照组的 DI 为 0.0698±0.0224，DM 红细胞的 DI 为 0.0645±0.03，DR 组为 0.0635±0.028 (p<0.001)。 DI 与红细胞基础长度呈负相关 (p = 0.02)。与正常健康对照相比，DM 和 DR 患者的 RBC DI 显着降低。因此，双光镊方法可以可靠地用于评估红细胞变形性。
A pilot cross sectional study was conducted to investigate the role of red blood cells (RBC) deformability in type 2 diabetes mellitus (T2DM) without and with diabetic retinopathy (DR) using a dual optical tweezers stretching technique. A dual optical tweezers was made by splitting and recombining a single Nd:YAG laser beam. RBCs were trapped directly (i.e., without microbead handles) in the dual optical tweezers where they were observed to adopt a “side-on” orientation. RBC initial and final lengths after stretching were measured by digital video microscopy, and a Deformability index (DI) calculated. Blood from 8 healthy controls, 5 T2DM and 7 DR patients with respective mean age of 52.4yrs, 51.6 yrs and 52 yrs was analysed. Initial average length of RBCs for control group was 8.45 ± 0.25 μm, 8.68 ± 0.49 μm for DM RBCs and 8.82 ± 0.32 μm for DR RBCs (p < 0.001). The DI for control group was 0.0698 ± 0.0224, and that for DM RBCs was 0.0645 ± 0.03 and 0.0635 ± 0.028 (p < 0.001) for DR group. DI was inversely related to basal length of RBCs (p = 0.02). DI of RBC from DM and DR patients was significantly lower in comparison with normal healthy controls. A dual optical tweezers method can hence be reliably used to assess RBC deformability.