Identification of Drosophila type II neuroblast lineages containing transit amplifying ganglion mother cells
Identification of Drosophila type II neuroblast lineages containing transit amplifying ganglion mother cells
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DOI：
10.1002/dneu.20648
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发表时间：
2008-08-01
影响因子：
3
通讯作者：
Doe, Chris Q.
中科院分区：
文献类型：
--
作者：
Boone, Jason Q.;Doe, Chris Q.
哺乳动物的神经干细胞产生能扩增神经元群体的转运扩增祖细胞，但这些类型的祖细胞尚未在果蝇中进行研究。果蝇幼虫的大脑每个脑叶含有大约100个神经干细胞（神经母细胞），这些神经干细胞被认为是从较小的神经节母细胞（GMC）中萌芽出来的，每个神经节母细胞产生两个有丝分裂后的神经元。在这里，我们使用分子标记和克隆分析，以确定一个新的神经母细胞系含有“过境扩增GMCs”（TA-GMCs）。TA-GMC在几个方面与典型GMC不同：每个TA-GMC具有核Deadpan，细胞质Prospero，在有丝分裂时形成Prospero新月，并产生多达10个神经元;典型GMC缺乏Deadpan，具有核Prospero，在有丝分裂时缺乏Prospero新月，并产生两个神经元。我们的结论是，至少有两种类型的神经母细胞谱系：I型谱系GMCs产生两个神经元，和II型谱系TA-GMCs有更长的谱系。II型谱系允许比I型谱系更快地产生更多的神经元，这在快速发育的生物体如果蝇中可能是有利的。(C)2008 Wiley Periodicals，Inc.
mammalian neural stem cells generate transit amplifying progenitors that expand the neuronal population, but these type of progenitors have not been studied in Drosophila. The Drosophila larval brain contains similar to 100 neural stem cells (neuroblasts) per brain lobe, which are thought to bud off smaller ganglion mother cells (GMCs) that each produce two post-mitotic neurons. Here, we use molecular markers and clonal analysis to identify a novel neuroblast cell lineage containing "transit amplifying GMCs" (TA-GMCs). TA-GMCs differ from canonical GMCs in several ways: each TA-GMC has nuclear Deadpan, cytoplasmic Prospero, forms Prospero crescents at mitosis, and generates up to 10 neurons; canonical GMCs lack Deadpan, have nuclear Prospero, lack Prospero crescents at mitosis, and generate two neurons. We conclude that there are at least two types of neuroblast lineages: a Type I lineage where GMCs generate two neurons, and a type II lineage where TA-GMCs have longer lineages. Type II lineages allow more neurons to be produced faster than Type I lineages, which may be advantageous in a rapidly developing organism like Drosophila. (C) 2008 Wiley Periodicals, Inc.