The use of immunofluorescence microscopy and enzyme-linked immunosorbent assay as complementary techniques for protein identification in artists' materials
The use of immunofluorescence microscopy and enzyme-linked immunosorbent assay as complementary techniques for protein identification in artists' materials
复制标题
DOI：
10.1179/019713606806112522
复制
发表时间：
2006-06-01
影响因子：
0.5
通讯作者：
Quick, Michael
中科院分区：
文献类型：
--
作者：
Heginbotham, Arlen;Millay, Victoria;Quick, Michael
与分析艺术品的传统方法相比，基于抗体的免疫学方法识别基于蛋白质的材料具有几个优势。这些技术能够很容易地区分不同的蛋白质类型(例如，胶原、卵清蛋白和酪蛋白)，还能够明确地确定蛋白质的生物来源(例如，牛胶原、兔胶原和羊胶原)。酶联免疫吸附分析技术灵敏度高，检测限在1毫微克以下，且相对简单、经济。此外，免疫荧光显微镜提供了在嵌入的横截面中空间分辨目标蛋白的可能性。本文介绍了一个案例研究，在分析安德烈-查尔斯·布勒(Andre-Charles Boulle)收藏的J·保罗·盖蒂博物馆(J.Paul Getty Museum)收藏的17世纪法国重要橱柜时，这些互补方法成功地用于蛋清蛋白的鉴定。作者认为，酶联免疫吸附试验和免疫荧光显微镜有可能成为保护科学实验室的常规分析工具。
Antibody-based immunological approaches to identifying protein-based materials offer several advantages over traditional methods of analyzing works of art. These techniques are able to easily distinguish different protein types (e.g., collagen vs. ovalbumin vs. casein), and also to determine unambiguously the biological source of the protein (e.g., bovine collagen vs. rabbit collagen vs. sheep collagen). The technique of enzyme-linked immunosorbent assay is highly sensitive, with detection limits below one nanogram, and is relatively simple and cost-effective. In addition, immunofluorescence microscopy offers the possibility of spatially resolving target proteins in embedded cross sections. This paper presents a case study in which these complementary methods were successfully employed for the identification of egg albumin in the analysis of an important 17th-century French cabinet by Andre-Charles Boulle in the collection of the J. Paul Getty Museum. The authors suggest that enzyme-linked immunosorbent assay and immunofluorescence microscopy have the potential to become routine analytical tools in conservation science laboratories.