Visualization of plasma membrane compartmentalization with patterned lipid bilayers
Visualization of plasma membrane compartmentalization with patterned lipid bilayers
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DOI：
10.1073/pnas.0403835101
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发表时间：
2004-09-21
影响因子：
11.1
通讯作者：
Baird, B
中科院分区：
文献类型：
--
作者：
Wu, M;Holowka, D;Baird, B
含有配体脂质的微米大小的图案化脂质双层用于控制受体簇的位置和大小，并能够直接可视化细胞成分的结构重组。在 Fcepsilon 受体 I（IgE 的肥大细胞受体）浓缩和共定位酪氨酸磷酸化活性之后，锚定在质膜内叶的 Lyn 激酶和其他蛋白质在依赖于肌动蛋白聚合的过程中选择性地与受体簇一起重新分布。令人惊讶的是，特征性地与脂筏相关的外部小叶成分并未可检测地与这些内部小叶成分共同分布。使用图案化表面的细胞激活提供了对细胞膜结构组织的独特见解，揭示了脂筏内部和外部小叶成分的动态、大规模解偶联。
Micrometer-size patterned lipid bilayers containing liganded lipids are used to control the location and size of receptor clusters and enable direct visualization of structural reorganization of cellular components. Subsequent to concentration of Fcepsilon receptor I, the mast cell receptor for IgE, and colocalized tyrosine phosphorylation activity, Lyn kinase and other proteins anchored to the inner leaflet of the plasma membrane redistribute selectively with the receptor clusters in a process that depends on actin polymerization. Surprisingly, outer leaflet components characteristically associated with lipid rafts do not detectably coredistribute with these inner leaflet components. Cell activation using patterned surfaces provides unique insights into cell membrane structural organization, revealing dynamic, large-scale uncoupling of inner and outer leaflet components of lipid rafts.