Mac-1+ cells are the predominant subset in the early hepatic lesions of mice infected with Francisella tularensis
Mac-1+ cells are the predominant subset in the early hepatic lesions of mice infected with Francisella tularensis
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DOI：
10.1128/iai.00868-06
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发表时间：
2006-12-01
影响因子：
3.1
通讯作者：
Benach, Jorge L.
中科院分区：
文献类型：
--
作者：
Rasmussen, John W.;Cello, Jeronimo;Benach, Jorge L.
实验性小鼠图拉热症早期肝脏病变的细胞成分尚无特异性标志物的特征。肝脏多发性肉芽肿坏死性病变的出现与血清丙氨酸转移酶和乳酸脱氢酶水平显著升高有关。通过特异性抗体检测到的土拉氏弗朗西斯氏菌，在接种后第一天就可以被发现，并在接种后5天与皮损相关。这些病变变得坏死，并有一些原位细胞凋亡的证据。病变不含B、T或NK细胞。相反，病变主要由与细菌相关的两个Mac-1(+)细胞亚群组成。GR-L(+)、Mac-1(+)未成熟髓系细胞和主要组织相容性复合体H-阳性(MHC-II+)Mac-1+巨噬细胞是肉芽肿中最丰富的细胞表型，可能是控制早期感染的主要因素。我们的研究结果表明，图拉氏丝虫与Gr-1(+)Mac-1(+)和MHC-II+Mac-1(+)细胞共存的肝脏病变发生在早期。
The cell composition of early hepatic lesions of experimental murine tularemia has not been characterized with specific markers. The appearance of multiple granulomatous-necrotic lesions in the liver correlates with a marked increase in the levels of serum alanine transferase and lactate dehydrogenase. Francisella tularensis, detected by specific antibodies, can be first noted by day I and becomes associated with the lesions by 5 days postinoculation. These lesions become necrotic, with some evidence of in situ apoptosis. The lesions do not contain B, T, or NK cells. Rather, the lesions are largely composed of two subpopulations of Mac-1(+) cells that are associated with the bacteria. Gr-l(+) Mac-1(+) immature myeloid cells and major histocompatibility complex class H-positive (MHC-II+) Mac-1+ macrophages were the most abundant cell phenotypes found in the granuloma and are likely major contributors in controlling the infection in its early stages. Our findings have shown that there is an early development of hepatic lesions where F. tularensis colocalizes with both Gr-1(+) Mac-1(+) and MHC-II+ Mac-1(+) cells.