E-cadherin and ZEB2 modulate trophoblast cell differentiation during placental development in pigs
E-cadherin and ZEB2 modulate trophoblast cell differentiation during placental development in pigs
复制标题
E-钙粘蛋白和ZEB2调节猪胎盘发育过程中的滋养层细胞分化
DOI：
10.1530/rep-17-0254
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发表时间：
2017-12-01
期刊：
影响因子：
3.8
通讯作者：
Yu, Mei
中科院分区：
文献类型：
--
作者：
Hong, Linjun;Han, Kun;Yu, Mei
在猪胎盘发育过程中，胎盘皱襞内的滋养层细胞分化为两种不同形态的细胞是重要的事件之一。结果表明，妊娠26 d时，约克郡猪和梅山猪的滋养层细胞形态相似，妊娠50 d时，位于胎盘皱襞顶部的滋养层细胞变为高柱状，位于胎盘皱襞底部的滋养层细胞变为立方形。妊娠95 d时，梅山猪的滋养层细胞全部变为立方形，而约克郡猪胎盘皱襞顶部的滋养层细胞仍为柱状。高柱状滋养层细胞共表达E-cadherin和β-catenin，而立方细胞共表达E-cadherin和β-catenin。因此，E-cadherin抑制子ZEB 2和E-cadherin在两个品种的两种滋养层细胞中的表达模式呈负相关。此外，电泳迁移率变动分析表明，结合ZEB 2的E-钙粘蛋白启动子在核提取物从猪胎盘组织。这些发现表明，在猪胎盘发育过程中，滋养层细胞分化的ZEB 2依赖机制。
It is one of the important events that trophoblast cells within the placental folds differentiate into two types that differ in cell shape during placental development in pigs. This study showed that all the trophoblast cells were of similar shape between Yorkshire and Chinese Meishan pigs on day 26 of gestation; thereafter, the trophoblast cells located at the top of the placental folds became high columnar, while those cells at the base of the placental folds were cuboidal on day 50 of gestation. Additionally, on day 95 of gestation, all the trophoblast cells in Meishan pigs became cuboidal, but the trophoblast cells located at the top of the placental folds in Yorkshire pigs still remained columnar. The membranous E-cadherin and beta-catenin were strongly co-expressed by the high columnar trophoblast cells but very weakly expressed by those cuboidal cells. Consistently, the expression pattern of ZEB2, the E-cadherin repressor, was inversely correlated with that of E-cadherin in the two types of trophoblast cells in the two breeds. Furthermore, electrophoretic mobility shift assays demonstrated the binding of ZEB2 to the E-cadherin promoter in nuclear extracts from porcine placental tissue. These findings suggest a ZEB2-dependent mechanism of trophoblast cell differentiation during placental development in pigs.