BpAP1 directly regulates BpDEF to promote male inflorescence formation in Betula platyphylla x B. pendula
BpAP1 directly regulates BpDEF to promote male inflorescence formation in Betula platyphylla x B. pendula
复制标题
BpAP1直接调节BpDEF促进白桦雄花序形成
DOI：
10.1093/treephys/tpz021
复制
发表时间：
2019
期刊：
影响因子：
4
通讯作者：
Chen Su
中科院分区：
文献类型：
--
作者：
Wang Shuo;Huang Haijiao;Han Rui;Chen Jiying;Jiang Jing;Li Huiyu;Liu Guifeng;Chen Su
植物开花是一个受复杂遗传控制的重要过程，AP 1是植物从营养生长向生殖生长转变的组织者和开关。在前期的研究中，我们发现BpAP 1基因的过表达显著促进了白桦（Betula platyphlla×B）雄花序的形成。pendula）。本研究旨在探讨BpAP 1在白桦雄花序形成过程中的分子调控机制。我们发现BpAP 1的过表达影响了许多开花相关基因的表达，其中对B类MADS-box基因的影响最为显著。构建了BpAP 1介导的基因调控网络，并最终预测B类基因BpDEF为BpAP 1的关键靶基因。染色质免疫沉淀结果表明，BpAP 1在雄花序形成过程中可直接调控BpDEF。酵母单杂交试验及其在烟草中的验证结果表明，BpAP 1通过与称为CArG盒的共有DNA序列结合来调节BpDEF。BpDEF基因功能分析表明，BpDEF基因可能在性别决定中起作用，尤其是对白桦雄花序的身份起决定作用。我们的研究结果为我们了解BpAP 1在桦树雄花序形成过程中的分子机制提供了有价值的线索。
Flowering is a crucial process for plants that is under complex genetic control.AP1acts as an organizer and a switch for the transition from vegetative to reproductive growth. In our previous study, we found that overexpression ofBpAP1significantly promoted the formation of male inflorescence in birch (Betula platyphlla×B. pendula). In this study, we aimed at investigating the molecular regulatory mechanism ofBpAP1during the process of male inflorescence formation in birch. We found that overexpression ofBpAP1affected the expression of many flowering-related genes, and had significant effect on B class MADS-box genes. A BpAP1-mediated gene regulatory network was constructed and B class geneBpDEFwas finally predicted as a key target gene of BpAP1. Chromatin immunoprecipitation results indicated that BpAP1 could directly regulateBpDEFduring the process of male inflorescence formation. Yeast one-hybrid assays and its validation in tobacco results suggested that BpAP1 regulatedBpDEFvia binding to a consensus DNA sequence known as CArG box. Gene function analysis ofBpDEFindicated thatBpDEFmay function in sex-determination, and in particular specify the identity of male inflorescence in birch. Our results provide valuable clues for our understanding of the molecular mechanism ofBpAP1during the process of male inflorescence formation in birch.