Regulation of expression of the myo-inositol monophosphatase 1 gene in osmoregulatory tissues of the European eel Anguilla anguilla after seawater acclimation.
Regulation of expression of the myo-inositol monophosphatase 1 gene in osmoregulatory tissues of the European eel Anguilla anguilla after seawater acclimation.
复制标题
海水驯化后欧洲鳗鱼渗透调节组织中肌醇单磷酸酶 1 基因表达的调节。
DOI：
10.1111/j.1749-6632.2009.04457.x
复制
发表时间：
2009
影响因子：
5.2
通讯作者：
Kalujnaia S
中科院分区：
文献类型：
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作者：
Kalujnaia S
本实验室先前的微阵列研究鉴定了从淡水（FW）转移到海水（SW）后在“银”鳗鱼中差异表达的许多基因。已经鉴定出一组与哺乳动物细胞中某些已知渗透调节剂的合成、加工和转运相关的基因。一个与渗透压产生有关的基因是肌肌醇单磷酸酶（IMPA 1）。本研究的目的是比较IMPA 1的表达的主要调节组织（肠，鳃，肾）之间的FW和SW环境中移动的鱼。在鳗鱼转移到SW后6 h，在任何组织中均未观察到IMPA 1基因表达的差异;然而，在适应2天后，在肾脏和鳃中分别发现mRNA表达增加了1.9倍和2.5倍。这些升高的水平在SW转移后维持长达5个月（分别为4.9倍和3.4倍）。在肠中未检测到IMPA 1 mRNA表达。Western blot分析证实，IMPA 1蛋白在鳃中上调，但在SW转移后5个月的肾脏中没有检测到蛋白丰度的变化。我们的研究揭示了IMPA 1在欧洲鳗鱼盐度适应中的潜在作用。
Previous microarray studies in our laboratory identified a number of genes that were differentially expressed in “silver” eels after transfer from freshwater (FW) to seawater (SW). A group of genes, which are related to the synthesis, processing, and transport of certain known osmolytes in mammalian cells, have been identified. One gene implicated with osmolyte production is myo‐inositol monophosphatase (IMPA1). The aim of this study was to compare the expression of IMPA1 in the major osmoregulatory tissues (intestine, gill, and kidney) as fish move between FW and SW environments. No difference in IMPA1 gene expression was observed in any tissues 6 h after eel transfer to SW; however, after 2 days acclimation, a 1.9‐ and a 2.5‐fold increase in mRNA expression was found in kidney and gill, respectively. These elevated levels were maintained for up to 5 months (4.9‐ and 3.4‐fold, respectively) after SW transfer. No IMPA1 mRNA expression was detected in the intestine. Western blot analysis confirmed the IMPA1 protein was upregulated in the gill, but no changes in protein abundance were detected in the kidney 5 months after SW transfer. Our studies have revealed a potential role for IMPA1 in salinity adaptation in the European eel.