CHLOROPHYLL A/B-BINDING PROTEINS, PIGMENT CONVERSIONS, AND EARLY LIGHT-INDUCED PROTEINS IN A CHLOROPHYLL B-LESS BARLEY MUTANT
CHLOROPHYLL A/B-BINDING PROTEINS, PIGMENT CONVERSIONS, AND EARLY LIGHT-INDUCED PROTEINS IN A CHLOROPHYLL B-LESS BARLEY MUTANT
复制标题
DOI：
10.1104/pp.107.3.873
复制
发表时间：
1995-03-01
期刊：
影响因子：
7.4
通讯作者：
JANSSON, S
中科院分区：
文献类型：
--
作者：
KROL, M;SPANGFORT, MD;JANSSON, S
已经针对源自不同植物光捕获叶绿素 a/b 结合 (LHC) 蛋白的一级序列的合成肽产生了单特异性多克隆抗体。与其他单特异性抗体一起，这些抗体被用来定量在正常和间歇光 (1mL) 下生长的野生型和氯 12 大麦 (Hordeum vulgare L.) 中 10 种不同的 LHC 蛋白的水平。在正常光照下生长的 Chlorina 12 缺乏 Lhcb1（I 型 LHC II）和 Lhcb6 (CP24)，并且 Lhcb2、Lhcb3（II 型和 III 型 LHC II）和 Lhcb4 (CP 29) 的量减少。在1mL下生长的Chlorina f2缺乏所有LHC蛋白，而野生型1mL植物含有Lhcb5 (CP 26)和少量Lhcb2。 lorina f2 ImL 类囊体组织成大的平行阵列，但野生型1mL 类囊体具有贴壁区域，表明Lhcb5 在基粒堆积中可能发挥作用。在1mL下生长的Chlorina f2含有大量的紫黄质（2-3/反应中心），代表与LHC蛋白不相关的光可转化叶黄素循环色素池。 Chlorina f2 和在 1mL 下生长的植物还含有通过蛋白质印迹监测的早期光诱导蛋白 (ELIP)。在 1 小时的强光处理期间，ELIP 和叶黄素循环色素的水平均有所增加，但 LHC 蛋白没有积累。这些数据与 ELIP 是色素结合蛋白的假设一致，我们建议 ELIP 结合光转换叶黄素并在光应激条件下取代“正常”LHC 蛋白。
Monospecific polyclonal antibodies have been raised against synthetic peptides derived from the primary sequences from different plant light-harvesting Chl a/b-binding (LHC) proteins. Together with other monospecific antibodies, these were used to quantify the levels of the 10 different LHC proteins in wild-type and chlorina 12 barley (Hordeum vulgare L.), grown under normal and intermittent light (ImL). Chlorina 12, grown under normal light, lacked Lhcb1 (type I LHC II) and Lhcb6 (CP24) and had reduced amounts of Lhcb2, Lhcb3 (types II and III LHC II), and Lhcb4 (CP 29). Chlorina f2 grown under ImL lacked all LHC proteins, whereas wild-type ImL plants contained Lhcb5 (CP 26) and a small amount of Lhcb2. The chlorina f2 ImL thylakoids were organized in large parallel arrays, but wild-type ImL thylakoids had appressed regions, indicating a possible role for Lhcb5 in grana stacking. Chlorina f2 grown under ImL contained considerable amounts of violaxanthin (2-3/reaction center), representing a pool of phototransformable xanthophyll cycle pigments not associated with LHC proteins. Chlorina f2 and the plants grown under ImL also contained early light-induced proteins (ELIPs) as monitored by western blotting. The levels of both ELIPs and xanthophyll cycle pigments increased during a 1 h of high light treatment, without accumulation of LHC proteins. These data are consistent with the hypothesis that ELIPs are pigment-binding proteins, and we suggest that ELIPs bind photoconvertible xanthophylls and replace ''normal'' LHC proteins under conditions of light stress.