Label-free immunosensing for alpha-fetoprotein in human plasma using surface plasmon resonance.
Label-free immunosensing for alpha-fetoprotein in human plasma using surface plasmon resonance.
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DOI：
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发表时间：
2007
影响因子：
2.9
通讯作者：
Y. Teramura;H. Iwata
中科院分区：
文献类型：
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作者：
Y. Teramura;H. Iwata
在这项研究中，我们试图开发一种基于表面等离子体共振(SPR)的免疫分析传感器，以在纳克水平检测人血浆中的甲胎蛋白(AFP)，以满足肝细胞肿瘤临床诊断的需要。利用三甘醇(TEG)和端羧基六甘醇(HEG)自组装单分子膜(SAM)抑制等离子体组分在传感器表面的非特异性吸附。在该系统中，甲胎蛋白的检测采用夹心式免疫分析法，使用两种抗体，即一抗和二抗。通过应用针对第二抗体的抗体(多克隆)，SPR信号漂移进一步增强。利用该方法，SPR信号被高度增强，因此可以很容易地检测到纳克级(ng/ml)的AFP，并具有较高的信噪比，这是临床诊断所必需的。预计我们的基于SPR的免疫分析方法也可以应用于检测其他几种存在于人体血液中的低浓度肿瘤标志物。
In this study, we attempted to develop a surface plasmon resonance (SPR)-based immunoassay sensor to detect alpha-fetoprotein (AFP) in human plasma at the nanogram level, as is required for clinical diagnosis of hepatocellular tumors. A self-assembled monolayer (SAM) surface of tri(ethylene glycol) (TEG) and carboxyl group-terminated hexa(ethylene glycol) (HEG) was employed to suppress the nonspecific adsorption of plasma components onto the sensor surface. AFP was detected by a sandwich-type immunoassay using two kinds of antibodies, primary and secondary, in this system. The SPR signal shift was further enhanced by applying an antibody (polyclonal) against the second antibody. With this method, the SPR signals were highly intensified, and so nanogram levels (ng/ml) of AFP could be easily detected with a high signal/noise ratio, as is necessary for clinical diagnosis. It is expected that our SPR-based immunoassay method can also be applicable to the detection of several other tumor markers that are present in low concentrations in human blood.