Role of serum matrix metalloproteinase in the diagnosis of gastric cancer
Role of serum matrix metalloproteinase in the diagnosis of gastric cancer
复制标题
血清基质金属蛋白酶在胃癌诊断中的作用
DOI：
10.12669/pjms.36.5.2059
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发表时间：
2020-07-01
影响因子：
2.2
通讯作者：
Yao，Bei
中科院分区：
文献类型：
--
作者：
Liu，Jian;Zhou，Liya;Yao，Bei
目的：探讨基质金属蛋白酶（MMP）抗体芯片在胃癌诊断中的临床应用价值。研究方法：在这项前瞻性研究中，在2017年11月至2018年7月期间收集了GC（n=66）和非肿瘤性胃病（NGD; n=34）患者的血清样本。采用MMP芯片对10种MMP相关蛋白进行定量检测，并对两组进行比较。结果如下：胃癌组血清MMPs 3、8、9及组织金属蛋白酶抑制剂（TIMPs）1、2水平均显著高于正常对照组（p<0.05）。用于诊断GC的10种MMP蛋白的曲线下面积（AUC）在0.500和0.658之间变化。所有MMP的总AUC为0.897（95% CI：0.837-0.957）。5种MMPs（MMPs 3、8、9和TIMPs 1和2）诊断GC的总AUC为0.821（95% CI：0.733-0.909）。此外，10因素和5因素预测模型对早期GC具有良好的诊断能力，AUC分别为0.865（95%CI：0.753-0.977）和0.749（95%CI：0.600-0.898）。结论：应用蛋白芯片技术检测多种血清基质金属蛋白酶有望成为胃癌早期诊断和筛查的一种新的无创性工具。
Objective: To determine the clinical value of a matrix metalloproteinase (MMP) antibody array in diagnosing gastric cancer (GC). Methods: In this prospective study, serum samples of patients with GC (n=66) and non-neoplastic gastric disease (NGD; n=34) were collected between November 2017 and July 2018. The quantitative measurement of 10 MMP-related proteins was done using MMP arrays and compared between the two groups. Results: The serum levels of MMPs 3, 8, 9 and tissue inhibitor of metalloproteinases (TIMPs) 1 and 2 were significantly higher in the GC group than in the NGD group (p<0.05). The area under curve (AUC) of the 10 MMP proteins for the diagnosis of GC varied between 0.500 and 0.658. The total AUC of all MMPs was 0.897 (95% CI: 0.837-0.957). The total AUC of the five MMPs (MMPs 3, 8, 9, and TIMPs 1 and 2) was 0.821 (95% CI: 0.733-0.909) for diagnosing GC. Also, the 10-factor and 5-factor predictive models had good diagnostic ability for early GC with an AUC of 0.865 (95% CI: 0.753-0.977) and 0.749 (95% CI: 0.600-0.898), respectively. Conclusions: The detection of multiple serum MMPs with protein biochip technology is promising to be used as a novel non-invasive tool for facilitating early diagnosis or screening of GC.