H2S-activatable near-infrared afterglow luminescent probes for sensitive molecular imaging in vivo
H2S-activatable near-infrared afterglow luminescent probes for sensitive molecular imaging in vivo
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DOI：
10.1038/s41467-020-14307-y
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发表时间：
2020-01-23
影响因子：
16.6
通讯作者：
Ye, Deju
中科院分区：
文献类型：
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作者：
Wu, Luyan;Ishigaki, Yusuke;Ye, Deju
具有高信号背景比的余辉发光探针显示出用于体内成像的前景;然而，可以选择性地递送到靶位点并开启余辉发光的这种探针仍然有限。我们优化了有机电致变色材料，并将其整合到近红外光敏剂（2，3-萘二甲双（三己基硅氧基）硅和聚[2-甲氧基-5-（2-乙基己氧基）-1，4-苯撑乙烯]）纳米粒子中，开发了H2S激活的近红外余辉探针（F1（2+）-ANP）。F1（2+）-ANP显示出对H2S的快速反应速率（1563 +/-141 M（-1）s（-1））和大的余辉开启比率（类似于122倍），使得能够高灵敏度和高特异性地测量来自健康人、肝癌或结直肠癌患者的血液中的H2S浓度。我们进一步构建了一种肝肿瘤靶向和H（2）可激活的余辉探针（F1（2+）-ANP-Gal），用于微小皮下HepG（2）肿瘤的非侵入性实时成像（
Afterglow luminescent probes with high signal-to-background ratio show promise for in vivo imaging; however, such probes that can be selectively delivered into target sites and switch on afterglow luminescence remain limited. We optimize an organic electrochromic material and integrate it into near-infrared (NIR) photosensitizer (silicon 2,3-naphthalocyanine bis( trihexylsilyloxide) and (poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylenevinylene]) containing nanoparticles, developing an H2S-activatable NIR afterglow probe (F1(2+)-ANP). F1(2+)-ANP displays a fast reaction rate (1563 +/- 141M(-1) s(-1)) and large afterglow turn-on ratio (similar to 122-fold) toward H2S, enabling high-sensitivity and -specificity measurement of H2S concentration in bloods from healthy persons, hepatic or colorectal cancer patients. We further construct a hepatic-tumor- targeting and H(2)Sactivatable afterglow probe (F1(2+)-ANP-Gal) for noninvasive, real-time imaging of tiny subcutaneous HepG(2) tumors (