Photonic Molecularly Imprinted Polymer Film for the Detection of Testosterone in Aqueous Samples
Photonic Molecularly Imprinted Polymer Film for the Detection of Testosterone in Aqueous Samples
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DOI：
10.3390/polym10040349
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发表时间：
2018-04-01
期刊：
影响因子：
5
通讯作者：
de Cortalezzi, Maria Fidalgo
中科院分区：
文献类型：
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作者：
Kadhem, Abbas J.;Xiang, Shuting;de Cortalezzi, Maria Fidalgo
由于在环境和生理样品中相关的低浓度水平，检测水溶液中的睾酮是一项艰巨的任务。目前的分析方法昂贵和/或复杂。为了解决这个问题，我们制作了一个分子印迹聚合物（MIP）光子膜检测水中的睾酮。使用胶体晶体作为孔形态的模板获得的膜。使用平均直径为330 nm的单分散二氧化硅颗粒通过垂直沉积获得胶体晶体。将丙烯酸溶液与睾酮作为印迹模板渗透到胶体晶体中并通过本体聚合进行聚合;然后通过酸蚀刻除去颗粒，并通过合适的溶剂洗脱睾酮。通过红外光谱、溶胀实验和显微镜对材料进行了表征;通过平衡再结合、动力学和重复使用实验对分子印迹聚合物进行了研究。结果表明，该分子印迹聚合物对模板分子具有选择性，平衡时间为30 min，使用和再生至少6次后仍保持稳定。孵育后，膜的反射光谱显示布拉格衍射峰的位移，其与5-100 ppb范围内的睾酮浓度相关。
The detection of testosterone in aqueous solutions is a difficult task due to the low concentration levels that are relevant in environmental and physiological samples. Current analytical methods are expensive and/or complex. To address this issue, we fabricated a molecularly imprinted polymer (MIP) photonic film for the detection of testosterone in water. The films were obtained using colloidal crystals as templates for the pore morphology. Monodispersed silica particles with an average diameter 330 nm were used to obtain the colloidal crystal by vertical deposition. A solution of acrylic acid with testosterone as the imprinted template was infiltrated in the colloidal crystal and polymerized via bulk polymerization; the particles were then removed by acid etching and the testosterone eluted by a suitable solvent. The material was characterized by FTIR, swelling experiments and microscopy; MIPs were investigated by equilibrium rebinding, kinetics and reuse experiments. The results showed that the MIPs exhibited selectivity to the template, a 30-min equilibration time and stability after at least six cycles of use and regeneration. After incubation, the reflectance spectra of the films showed a shift of the Bragg diffraction peak that correlated with testosterone concentration in the 5-100 ppb range.