Inhibition by steroids of the uptake of potassium by capillaries isolated from rat brain.
Inhibition by steroids of the uptake of potassium by capillaries isolated from rat brain.
复制标题
类固醇抑制大鼠脑中分离的毛细血管对钾的吸收。
DOI：
10.1016/0006-2952(81)90084-8
复制
发表时间：
1981
影响因子：
5.8
通讯作者：
Goldstein,GW
中科院分区：
文献类型：
--
作者：
Chaplin,ER;Free,RG;Goldstein,GW
研究了类固醇对大鼠脑毛细血管摄取和释放K+的影响。哇巴因可明显抑制毛细血管对~（86）Rb ~+的摄取。哇巴因敏感的~（86）Rb ~+摄取部分被皮质酮抑制，抑制浓度为8 × 10− 4 M。抑制作用立即发作，在去除类固醇后迅速可逆。皮质酮不改变K+转运载体的亲和力或86 Rb+从毛细血管的被动流出。其他类固醇抑制哇巴因敏感的~（86）Rb+摄取与其脂溶性有关。这些特征表明，高剂量类固醇治疗可能对Na+，K+ ATP酶有直接的膜作用，而不是细胞功能的核介导的变化。
The effect of steroids on the uptake and release of K+by capillaries isolated from rat brain was investigated.86Rb+uptake was used as a transport analog of K+. The uptake of86Rb+by the capillaries was markedly inhibited by ouabain. The ouabain sensitive fraction of86Rb+uptake was inhibited by corticosterone with an I50of 8 × 10−4M. Inhibition was immediate in onset and rapidly reversible after removal of the steroid. Corticosterone did not alter the affinity of the transport carrier for K+or the passive efflux of86Rb+from the capillaries. Other steroids inhibited ouabain sensitive86Rb+uptake in relation to their lipid solubility. These features suggest a direct membrane action for high dose steroid therapy, possibly on Na+,K+ATPase, rather than a nuclear mediated change in cell function.