Complete nucleotide and deduced amino acid sequences of rat L‐type pyruvate kinase
Complete nucleotide and deduced amino acid sequences of rat L‐type pyruvate kinase
复制标题
大鼠L型丙酮酸激酶的完整核苷酸和推导的氨基酸序列
DOI：
10.1016/0014-5793(86)80138-7
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发表时间：
1986
期刊：
影响因子：
3.5
通讯作者：
J. Marie
中科院分区：
文献类型：
--
作者：
Y. Lone;M. Simon;A. Kahn;J. Marie
从碳水化合物诱导的大鼠肝脏cDNA文库中分离出L型丙酮酸激酶（PK-L）的四个重叠cDNA克隆。它们包含从第7个密码子开始的酶的所有编码序列和整个3 '-非翻译延伸直到poly（A）尾。通过引物延伸确定前7个密码子的序列和5 '非翻译区的序列。分析的PK‐L mRNA有19个5 '非翻译碱基，1629个编码碱基和1281个3'非翻译碱基，无poly（A）尾;它对应于较重的3.2 kb L型mRNA。磷酸化位点的密码子位于信使的5 '端。异常长的3 '-非翻译延伸包含与Sutcliffe等人[（1982）Proc.Natl. Acad. Sci. USA 79，4942 - 4946]。
Four overlapping cDNA clones for L‐type pyruvate kinase (PK‐L) were isolated from carbohydrate‐induced rat liver cDNA libraries. They contained all the coding sequence of the enzyme from the 7th codon and the entire 3'‐untranslated extension up to the poly(A) tail. The sequence of the first 7 codons and that of the 5'‐untranslated region were determined by primer extension. The analyzed PK‐L mRNA has 19 5'‐untranslated bases, 1629 coding bases and 1281 3'‐untranslated bases without the poly(A) tail; it corresponds to the heavier, 3.2 kb species of the L‐type mRNAs. The codons for the phosphorylatable site are, located at the 5'‐end of the messenger. The unusually long 3'‐untranslated extension contains a repetitive element complementary to the ‘brain‐specific’ identifier sequence described by Sutcliffe et al. [(1982) Proc. Natl. Acad. Sci. USA 79, 4942‐4946].