Helicobacter species ribosomal DNA in the pancreas, stomach and duodenum of pancreatic cancer patients
Helicobacter species ribosomal DNA in the pancreas, stomach and duodenum of pancreatic cancer patients
复制标题
DOI：
10.3748/wjg.v12.i19.3038
复制
发表时间：
2006-05-21
影响因子：
4.3
通讯作者：
Wadstrom, Torkel
中科院分区：
文献类型：
--
作者：
Nilsson, Hans-Olof;Stenram, Unne;Wadstrom, Torkel
目的：探讨胃癌和肠道幽门螺杆菌是否与胰腺癌有关。方法：外分泌型胰腺癌（n = 40）、神经内分泌癌（n = 14）、I型多发性内分泌瘤（n = 8）、慢性胰腺炎（n = 5）患者进行研究。其他良性胰腺疾病（n = 10）和正常胰腺标本（n = 7）作为对照。胰腺组织标本采用幽门螺杆菌特异性pcr分析，产物采用变性梯度电泳和dna测序进行鉴定。从一部分胰腺癌患者中，我们分析了胃和/或十二指肠组织以及胆囊和胆管组织。胆囊和胆囊标本作为对照。对胰腺癌患者的胃和十二指肠标本进行了调查，分析了一种胃幽门螺杆菌是否可能向胰腺扩散。采用拟杆菌特异性PCR对胰腺标本进行分析，以检测本地肠道微生物向病变胰腺的易位。结果：75%的外分泌癌患者、57%的神经内分泌癌患者、38%的多发性内分泌瘤患者和60%的慢性胰腺炎患者的胰腺（肿瘤和/或周围组织）中检测到幽门螺杆菌DNA。其他良性胰腺疾病及正常胰腺标本均为阴性。33%的患者胃十二指肠标本幽门螺杆菌阳性。令人惊讶的是，在60%的胃十二指肠阳性样本中发现了胆汁分枝杆菌。胆囊和胆总管标本均为幽门螺杆菌阴性。所有胰腺标本的拟杆菌pcr检测均为阴性。结论：在胰腺癌中常见的幽门螺杆菌DNA提示这种新出现的病原体可能在慢性胰腺炎和胰腺癌的发展中起作用。(c) 2006年WJG出版社。版权所有。
AIM: To determine whether gastric and enteric Helicobacter species are associated with pancreatic cancer.METHODS: Patients with exocrine pancreatic cancer (n = 40), neuroendocrine cancer (n = 14), multiple endocrine neoplasia type I (n = 8), and chronic pancreatitis (n = 5) were studied. Other benign pancreatic diseases (n = 10) and specimens of normal pancreas (n = 7) were included as controls. Pancreatic tissue specimens were analyzed by Helicobacter-specific PCR-assay and products were characterized by denaturing gradient electrophoresis and DNA-sequencing. From a subset of the pancreatic cancer patients, gastric and/or duodenal tissue as well as gallbladder and ductus choleclochus tissue were analyzed. Gallbladder and choleclochus samples were included as controls. Stomach and duodenum samples were investigated to analyze whether a gastric Helicobacter might disseminate to the pancreas in pancreatic cancer patients. Pancreatic specimens were analyzed by Bacteroides-specific PCR for detecting the translocation of indigenous gut microbes to the diseased pancreas.RESULTS: Helicobacter DNA was detected in pancreas (tumor and/or surrounding tissue) of 75% of patients with exocrine cancer, 57% of patients with neuroendocrine cancer, 38% of patients with multiple endocrine neoplasia, and 60% of patients with chronic pancreatitis. All samples from other benign pancreatic diseases and normal pancreas were negative. Thirty-three percent of the patients were Helicobacter positive in gastroduodenal specimens. Surprisingly, H. bilis was identified in 60% of the positive gastroduodenal samples. All gallbladder and ductus choledochus specimens were negative for helicobacter. Bacteroides PCR-assay was negative for all pancreatic samples.CONCLUSION: Helicobacter DNA commonly detected in pancreatic cancer suggests a possible role of the emerging pathogens in the development of chronic pancreatitis and pancreatic cancer. (c) 2006 The WJG Press. All rights reserved.