Transplantation of allogeneic bone marrow without graft-versus-host disease using total lymphoid irradiation.
Transplantation of allogeneic bone marrow without graft-versus-host disease using total lymphoid irradiation.
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DOI：
10.1084/jem.147.4.963
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发表时间：
1978-04-01
期刊：
影响因子：
--
通讯作者：
Strober S
中科院分区：
文献类型：
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作者：
Slavin S;Fuks Z;Kaplan HS;Strober S
将来自 C57BL/Ka (H-2b/b) 小鼠的骨髓 (BM) 和同种异体皮肤移植到经全淋巴照射 (TLI)、全身照射 (WBI) 或分次胸腺照射处理的 BALB/c (H-2d/d) 受体中。TLI 将同种异体皮肤移植物的存活时间延长至未治疗对照组的约五倍，并允许约 90% 的 BM 细胞永久植入。收件人。骨髓移植受者均未表现出移植物抗宿主病（GVHD）的临床症状（腹泻、体重减轻、驼背等）。另一方面，接受 WBI 和同种异体 BM 细胞的受者出现严重的临床 GVHD。后者大多数受者在骨髓移植后12天内死亡，95%在61天内死亡。尽管 TLI 保护 BALB/c 小鼠免受 BM 细胞诱导的 GVHD，但所有给予 TLI 和同种异体脾细胞的受体均出现致命的 GVHD。单独的胸腺照射略微延长了同种异体皮肤移植物的存活率，并且不允许同种异体骨髓移植。这些结果表明，TLI 可能是临床骨髓移植中的一种有用的治疗方案，因为这种形式的放射治疗在人类中广泛使用，并且几乎没有严重的副作用。
Bone marrow (BM) and skin allografts from C57BL/Ka (H-2b/b) mice were transplanted to BALB/c (H-2d/d) recipients treated with total lymphoid irradiation (TLI), whole-body irradiation (WBI), or fractionated thymic irradiation TLI prolonged skin allograft survival about five times as long as that in untreated controls, and allowed for permanent engraftment of BM cells in approximately equal to 90% of recipients. None of the BM recipients showed clinical signs of graft-versus-host disease (GVHD) (diarrhea, weight loss, hunched back, etc.). On the other hand, recipients given WBI and allogeneic BM cells developed severe clinical GVHD. The majority of the latter recipients died within 12 days after BM transplantation, and 95% died within 61 days. Although TLI protected BALB/c mice against GVHD induced by BM cells, all recipients given TLI and allogeneic spleen cells developed lethal GVHD. Thymic irradiation alone marginally prolonged skin allograft survival, and did not allow for allogeneic BM engraftment. These results suggest that TLI may be a useful regimen in clinical BM transplantation, since this form of radiotherapy is used extensively in humans and has few severe side effects.