Evaluation of rapid methods and novel indicators for assessing microbiological beach water quality
Evaluation of rapid methods and novel indicators for assessing microbiological beach water quality
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DOI：
10.1016/j.watres.2009.09.017
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发表时间：
2009-11-01
期刊：
影响因子：
12.8
通讯作者：
Weisberg, Stephen B.
中科院分区：
文献类型：
--
作者：
Griffith, John F.;Cao, Yiping;Weisberg, Stephen B.
为评估泳滩水质，已发展了一套广泛的以分子测量技术为基础的新测量方法和指标，但这些方法一般都是在开发这些方法的实验室之外进行有限的测试。在这里，我们评估了29种针对各种细菌、病毒和化学分析的检测方法，为方法开发人员提供了12个盲样，其中包括已知浓度的污水或鸥粪样品和阴性对照。对每种方法的检测目标生物的能力、阴性对照中信号的缺失以及重复之间的可重复性进行了评估。30种方法中只有6种在至少75%的样品中检测到它们的目标，而在阴性对照中一致地确定目标缺失。在定量方法中，Luminex检测的拟杆菌和肠球菌的QPCR可靠地鉴定出除一个样品外的所有含有人类粪便的样品，并且没有产生假阳性结果。在非定量方法中，肠球菌（esp）基因、拟杆菌属（Bacteroidales）人特异性标记物和基于培养的噬菌体是鉴定人粪便物质最可靠的方法。我们还发现，针对同一生物体的研究人员特定方法的变化通常会产生不同的结果。2009爱思唯尔有限公司版权所有。
A broad suite of new measurement methods and indicators based on molecular measurement technology have been developed to assess beach water quality, but they have generally been subjected to limited testing outside of the laboratory in which they were developed. Here we evaluated 29 assays targeting a variety of bacterial, viral, and chemical analytes by providing the method developers with twelve blind samples consisting of samples spiked with known concentration of sewage or gull guano and negative controls. Each method was evaluated with respect to its ability to detect the target organism, absence of signal in the negative controls and repeatability among replicates. Only six of the 30 methods detected their targets in at least 75% of the samples while consistently determining the absence of the target in the negative controls. Among quantitative methods, QPCR for Bacteroides thetaiotamicron and Enterococcus detected by Luminex reliably identified all but one sample containing human fecal material and produced no false positive results. Among non-quantitative methods, the Enterococcus esp gene, the Bacteroidales human specific marker and culture-based coliphage were the most reliable for identifying human fecal material. We also found that investigator-specific variations of methods targeting the same organism often produced different results. (C) 2009 Elsevier Ltd. All rights reserved.