IDENTIFICATION OF A GENE INDUCED BY GLUCOCORTICOIDS IN MURINE T-CELLS - A POTENTIAL-G PROTEIN-COUPLED RECEPTOR
IDENTIFICATION OF A GENE INDUCED BY GLUCOCORTICOIDS IN MURINE T-CELLS - A POTENTIAL-G PROTEIN-COUPLED RECEPTOR
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DOI：
10.1210/mend-5-9-1331
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发表时间：
1991-09-01
影响因子：
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通讯作者：
BOURGEOIS, S
中科院分区：
文献类型：
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作者：
HARRIGAN, MT;CAMPBELL, NF;BOURGEOIS, S
利用cDNA克隆技术，我们先前在小鼠T淋巴细胞中发现了一组由糖皮质激素和cAMP诱导的基因。我们在这里报道了其中一个克隆(克隆4.2)的序列，在这里被重新命名为糖皮质激素诱导受体(GIR)，它编码与G蛋白偶联的受体家族中的一个潜在的新成员。利用核糖核酸酶保护实验分离了该基因的几种不同形式的cDNAs，并证明其对应于淋巴细胞中的多种mRNAs。该克隆对应于GIR基因最丰富的形式，编码423个氨基酸的前体蛋白，推测信号肽由17个氨基酸组成。疏水图显示了七个疏水区的存在，与其他G蛋白偶联受体有显著的相似之处。GIR的配基目前尚不清楚，但该基因的预测蛋白产物与三种速激肽受体关系最为密切，即Neuromedin-K、Substance-P和Substance-K受体。
Using cDNA cloning techniques we previously identified a set of genes induced by glucocorticoids and cAMP in murine T-lymphocytes. We report here the sequence of one of these cDNA clones (clone 4.2), renamed here as glucocorticoid-induced receptor (GIR), which encodes a potential new member of the family of receptors that couple to G-proteins. Several different forms of cDNA for this gene were isolated and shown to correspond to multiple mRNA species in lymphoid cells using an RNase protection assay. The cDNA clone corresponding to the most abundant form of GIR mRNA encodes a precursor protein of 423 amino acids, with a putative signal peptide of 17 amino acids. A hydropathy plot reveals the presence of seven hydrophobic regions, with significant similarities to other G-protein-coupled receptors. The ligand for GIR is currently unknown, but the predicted protein product of this cDNA is most closely related to the three tachykinin receptors, the neuromedin-K, substance-P, and substance-K receptors.