Terpene arms race in the Seiridium cardinale - Cupressus sempervirens pathosystem.
Terpene arms race in the Seiridium cardinale - Cupressus sempervirens pathosystem.
复制标题
DOI：
10.1038/srep18954
复制
发表时间：
2016-01-22
影响因子：
4.6
通讯作者：
Peñuelas J
中科院分区：
文献类型：
--
作者：
Achotegui-Castells A;Della Rocca G;Llusià J;Danti R;Barberini S;Bouneb M;Simoni S;Michelozzi M;Peñuelas J
引起溃疡的红景天真菌是全球范围内对西伯利亚柏树的主要威胁。我们研究了接种红假单胞菌的耐溃烂和敏感柏树产生萜烯的情况，这些萜烯对真菌生长的影响，以及真菌对这些萜烯的防御性生物转化。所有受侵染的树木都产生了新萜类化合物，并强烈地诱导了萜类化合物的反应，但抗溃烂的树木的反应比感病的树木强。抑制真菌生长的体外试验表明，抗病树种的萜类化合物浓度比感病树种的抑制作用强。高度诱导的和去新生的萜类化合物表现出显著的抑制作用(超过杀菌剂的参照物)，并且具有高浓度依赖的抑制作用，而最丰富的萜类化合物具有低浓度依赖的抑制作用。红衣菌对三种萜类化合物进行了生物转化，即使在真菌菌丝体外，也能在其周围环境中对它们进行解毒。因此，我们的研究结果表明，萜类化合物不仅是半圆线虫有效利用的关键防御手段，而且也表明红衣藻已经为战斗做好了准备。
The canker-causing fungus Seiridium cardinale is the major threat to Cupressus sempervirens worldwide. We investigated the production of terpenes by canker-resistant and susceptible cypresses inoculated with S. cardinale, the effect of these terpenes on fungal growth, and the defensive biotransformation of the terpenes conducted by the fungus. All infected trees produced de novo terpenes and strongly induced terpenic responses, but the responses were stronger in the canker-resistant than the susceptible trees. In vitro tests for the inhibition of fungal growth indicated that the terpene concentrations of resistant trees were more inhibitory than those of susceptible trees. The highly induced and de novo terpenes exhibited substantial inhibition (more than a fungicide reference) and had a high concentration-dependent inhibition, whereas the most abundant terpenes had a low concentration-dependent inhibition. S. cardinale biotransformed three terpenes and was capable of detoxifying them even outside the fungal mycelium, in its immediate surrounding environment. Our results thus indicated that terpenes were key defences efficiently used by C. sempervirens, but also that S. cardinale is ready for the battle.