MASS ISOLATION OF PLANT CHROMOSOMES AND NUCLEI
MASS ISOLATION OF PLANT CHROMOSOMES AND NUCLEI
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DOI：
10.1007/bf00405195
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发表时间：
1983-01-01
期刊：
影响因子：
4.3
通讯作者：
DUDITS, D
中科院分区：
文献类型：
--
作者：
HADLACZKY, G;BISZTRAY, G;DUDITS, D
提出了一种从植物原生质体中大量分离中期染色体和细胞核的方法。使用小麦（Triticum monocaccum）和罂粟（Papaver somniferum）细胞培养物为单子叶和双子叶物种开发了分离程序。从部分同步的有丝分裂原生质体中分离中期染色体，而为了分离细胞核，则使用不同步的原生质体。光和电子显微镜研究表明，分离的染色体和细胞核保留了完整的形态。通过聚丙烯酰胺凝胶电泳对染色体蛋白进行了初步生化研究。由于分离的染色体和细胞核的纯度和高数量，给定的分离程序可以为结构和生化研究以及遗传操作提供有用的材料。
A method is presented for mass isolation of metaphase chromosomes and nuclei from plant protoplasts. The isolation procedure was developed for monocotyledonous and dicotyledonous species using wheat (Triticum monococcum) and poppy (Papaver somniferum) cell cultures. Metaphase chromosomes were isolated from partially synchronized mitotic protoplasts, while for the isolation of nuclei, unsynchronized protoplasts were used. Light and electron microscopic studies revealed that isolated chromosomes and nuclei preserved their intact morphology. A preliminary biochemical study of chromosomal proteins was made by polyacrylamide gel electrophoresis. Because of the purity and high quantity of isolated chromosomes and nuclei, the given isolation procedure can supply useful material for structural and biochemical studies, and for genetic manipulation.