Antennal SNMPs (sensor neuron membrane proteins) of lepidoptera define a unique family of invertebrate CD36-like proteins
Antennal SNMPs (sensor neuron membrane proteins) of lepidoptera define a unique family of invertebrate CD36-like proteins
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DOI：
10.1002/neu.1065
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发表时间：
2001-10-01
期刊：
影响因子：
--
通讯作者：
Vogt, RG
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文献类型：
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作者：
Rogers, ME;Krieger, J;Vogt, RG
SNMP1-Apol1是一种天蚕蛾触角特异蛋白，广泛表达并定位于性信息素特异性嗅觉神经元的感受器膜上。SNMP1-Apol因其嗅觉特异性表达、在OSNs中的定位、表达的发育时间以及与CD36膜结合受体蛋白家族的明显同源性而被认为在气味检测中发挥作用。在目前的研究中，SNMP1-Apol同源物被从家蚕、绿光夜蛾和六叶蛾中鉴定出来。这些物种具有触角mRNAs，编码氨基酸序列同源性在75%-80%之间的蛋白质；这些蛋白质统称为SNMP1。对先前鉴定并部分测序的第二个AL sexta SNMP同系物[Robertson等人：昆虫分子生物8：501-518,1999]进行了完全测序和鉴定。编码的蛋白与SNMP1蛋白的序列同源性只有26%-27%，因此被命名为SNMP2-Msex。利用该序列分别在果蝇和线虫基因组数据库中鉴定了14个和4个可能的同源物，从而极大地扩大了CD36家族在无脊椎动物谱系中的成员范围。尽管SNMP1-Msex和SNMP2-Msex的序列不同，但SNMP1-Msex和SNMP2-Msex的表达局限于OSNs，并与嗅觉功能的开始同时发生。这些发现表明，SNMPs在昆虫的气味检测中发挥着核心作用，CD36基因家族在动物门中广泛存在。SNMP是CD36家族中唯一被识别的神经元成员，因此将该基因家族的活动扩展到影响大脑功能和行为行为的角色。(C)2001年John Wiley&Sons，Inc.
SNMP1-Apol is an antennal-specific protein of the wild silk moth Antheraea polyphemus; the protein Is abundantly expressed and localized to the receptor membranes of sex-pheromone specific olfactory sensory neurons (OSNs). SNMP1-Apol is thought to function in odor detection based on its olfactory-specific expression, localization within OSNs, developmental time of expression, and apparent homology to the CD36 family of membrane-bound receptor proteins. In the current study, SNMP1-Apol homologues were identified from the moths Bombyx mori, Heliothis virescens, and Manduca sexta. These species posses antennal mRNAs encoding proteins with amino acid sequence identities ranging from 75-80%; these proteins are collectively designated SNMP1. A second AL sexta SNMP homologue, previously identified and partially sequenced [Robertson et al.: Insect Mol Biol 8:501-518, 1999] was fully sequenced and characterized. The encoded protein shares only 26-27% sequence identity with the SNMP1 proteins, and is thus designated SNMP2-Msex. The SNMP sequences were used to identify 14 and four possible homologues in Drosophila melanogaster and Caenorhabditis elegans genome databases, respectively; thus, greatly expanding CD36 family membership among the invertebrate lineages. Despite their sequence difference, SNMP1-Msex and SNMP2-Msex expression is localized to OSNs and occurs simultaneously with the onset of olfactory function. These findings suggest that SNMPs play a central role in odor detection in insects, and that the CD36 gene family is widely represented among animal phyla. The SNMPs are the only identified neuronal members of the CD36 family, and as such expand the activities of this gene family into roles influencing brain function and behavioral action. (C) 2001 John Wiley & Sons, Inc.