Two-Dimensional Liquid Chromatography-Tandem Mass Spectrometry Coupled with Isobaric Tags for Relative and Absolute Quantification (iTRAQ) Labeling Approach Revealed First Proteome Profiles of Pulmonary Alveolar Macrophages Infected with Porcine Reproductive and Respiratory Syndrome Virus
Two-Dimensional Liquid Chromatography-Tandem Mass Spectrometry Coupled with Isobaric Tags for Relative and Absolute Quantification (iTRAQ) Labeling Approach Revealed First Proteome Profiles of Pulmonary Alveolar Macrophages Infected with Porcine Reproductive and Respiratory Syndrome Virus
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DOI：
10.1021/pr201266z
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发表时间：
2012-05-01
影响因子：
4.4
通讯作者：
Jiang, Ping
中科院分区：
文献类型：
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作者：
Lu, Qi;Bai, Juan;Jiang, Ping
猪繁殖与呼吸综合征（PRRS）已在全球范围内肆虐养猪业近25年，其病毒（PRRSV）优先在肺泡巨噬细胞（PAM）中感染和复制。为了发现PRRSV感染PAM中的细胞蛋白反应，采用二维液相色谱-串联质谱结合同量异位素相对和绝对定量标签（iTRAQ）标记来定量鉴定PRRSV感染组和对照组之间的差异表达蛋白。共鉴定了160种PAM中感染后显著改变的细胞蛋白。这些差异表达的蛋白质与病毒结合、细胞结构、信号转导、细胞粘附等生物学过程有关，以及他们之间的互动这是第一份使用iTRAQ技术分析PRRSV感染PAM的细胞蛋白质谱的报告，该数据提供了重要信息，有助于了解宿主对PRRSV的反应，并确定PRRSV复制和发病机制的细胞要求。
Porcine reproductive and respiratory syndrome (PRRS) has devastated the pig industry worldwide for almost 25 years, and its virus (PRRSV) preferentially infects and replicates in pulmonary alveolar macrophages (PAMs). To discover cellular protein responses in PRRSV-infected PAMs, two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling was employed to quantitatively identify the differentially expressed proteins between the PRRSV-infected groups and the controls. A total of 160 cellular proteins in PAMs that were significantly altered post-infection were identified. These differentially expressed proteins are related to the biological processes of virus binding, cell structure, signal transduction, cell adhesion, etc., and their interactions. This is the first report that analyzed the cellular protein profile of PRRSV-infected PAMs using iTRAQ technology, and this data provides important information to help understand the host response to PRRSV and to define the cellular requirements for the underlying mechanism of PRRSV replication and pathogenesis.