Effects of the acid polysaccharide fraction isolated from a cultivated Cordyceps sinensis on macrophages in vitro
Effects of the acid polysaccharide fraction isolated from a cultivated Cordyceps sinensis on macrophages in vitro
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培养冬虫夏草中分离的酸性多糖组分对体外巨噬细胞的影响
DOI：
10.1016/j.cellimm.2010.01.001
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发表时间：
2010-01-01
影响因子：
4.3
通讯作者：
Wang, Kongcheng
中科院分区：
文献类型：
--
作者：
Chen, Weixia;Zhang, Weiyun;Wang, Kongcheng
从人工虫草菌丝体中提取的酸性多糖组分（APSF）是一种水溶性多糖。本实验研究了APSF对小鼠巨噬细胞系RAW 264.7的调节作用。中性红吞噬实验和FITC-葡聚糖内吞实验表明，APSF能刺激巨噬细胞的吞噬功能。用Griess试剂测定培养上清中的亚硝酸盐含量，表明APSF处理后NO产量增加。RT-PCR和免疫细胞化学检测结果表明，APSF可促进诱导型一氧化氮合酶（iNOS）mRNA和蛋白的表达。Western blotting结果显示，APSF处理后巨噬细胞核内NF-κ B水平升高，提示APSF可能通过激活I κ B-NF-κ B通路来刺激巨噬细胞活性。(C)2010年爱思唯尔公司All rights reserved.
The acid polysaccharide fraction (APSF) extracted from the mycelia of cultivated Cordyceps sinensis is water-soluble polysaccharide. In this study we evaluated the modulating effects of APSF on murine macrophage cell line RAW264.7. Phagocytotic assay by neutral red and FITC-dextran internalization showed that APSF stimulated the phagocytosis of macrophages. The nitrite levels in the culture Supernatant determined using Griess reagent revealed the elevation of NO production after treatment with APSF. RT-PCR and immunocytochemistry assay indicated that APSF promoted both the mRNA and protein expressions of inducible nitric oxide synthase (iNOS). Furthermore, Western blotting demonstrated that NF-kappa B levels in nucleuses increased after APSF treatment, suggesting that APSF probably stimulated macrophage activities by activating the I kappa B-NF-kappa B pathway. (C) 2010 Elsevier Inc. All rights reserved.