Contribution of transglutaminases and their substrate proteins to the formation of cornified cell envelope in oral mucosal epithelium
Contribution of transglutaminases and their substrate proteins to the formation of cornified cell envelope in oral mucosal epithelium
复制标题
转谷氨酰胺酶及其底物蛋白对口腔粘膜上皮角质化细胞包膜形成的贡献
DOI：
10.1111/eos.12760
复制
发表时间：
2021
影响因子：
1.9
通讯作者：
Hasegawa Hiromasa
中科院分区：
文献类型：
--
作者：
Roy Rita Rani;Shimada Katsumitsu;Murakami Satoshi;Hasegawa Hiromasa
角质膜的形成对于角化上皮的最终分化至关重要。然而，在口腔上皮中皮质包膜形成的机制仍不清楚。本研究的目的是阐明角化和非角化口腔上皮细胞之间的角化膜相关蛋白和基因的分布和表达差异。我们用化学方法研究了19个角化和14个非角化口腔上皮样本中转氨酶1（TG 1）、转氨酶3（TG 3）及其底物蛋白外皮蛋白（IVL）、兜甲蛋白（LOR）和富含脯氨酸的小蛋白（SPR）的分布模式。使用石蜡包埋标本，通过真实的时间逆转录聚合酶链反应（RT-PCR）研究两种类型上皮中的TG 1和TG 3 mRNA水平。分析数据以确定参与皮质包膜形成的因素。我们证明了11种定位模式在角化和非角化口腔上皮细胞之间显示出统计学显著差异。在聚类分析中，这些因素明显地推动了两组的分离。角化口腔上皮中的TG 1 mRNA水平显著高于非角化口腔上皮。总之，特征分布的转氨酶及其底物和TG 1的mRNA水平可以调节角化口腔上皮中的皮质包膜形成，连同本文首次报道的TG 3的贡献。
Cornified envelope formation is crucial for the final differentiation of keratinized epithelium. However, the mechanisms of cornified envelope formation in the oral epithelium remain unclear. The aim of this study was to clarify the differences in the distribution and expression of cornified envelope related proteins and genes between keratinized and non‐keratinized oral epithelia. We immunohistochemically investigated the distribution patterns of transglutaminase 1 (TG1), transglutaminase 3 (TG3), and their substrate proteins involucrin (IVL), loricrin (LOR), and small proline rich proteins (SPRs), in 19 keratinized and 14 non‐keratinized oral epithelium samples. TG1 and TG3 mRNA levels were investigated in both types of epithelium by real time reverse transcription polymerase chain reaction (RT‐PCR) using paraffin‐embedded specimens. Data were analyzed to identify factors involved in cornified envelope formation. We demonstrate that 11 localization patterns show statistically significant differences between keratinized and non‐keratinized oral epithelia. These factors clearly drove the separation of the two groups during cluster analysis. TG1 mRNA levels in keratinized oral epithelium were significantly higher than those in non‐keratinized oral epithelium. In conclusion, the characteristic distribution of transglutaminases and their substrates and the mRNA levels of TG1 can regulate cornified envelope formation in keratinized oral epithelium, together with the contribution of TG3 first reported in this paper.