CYANINE DYE LABELING REAGENTS - CARBOXYMETHYLINDOCYANINE SUCCINIMIDYL ESTERS
CYANINE DYE LABELING REAGENTS - CARBOXYMETHYLINDOCYANINE SUCCINIMIDYL ESTERS
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DOI：
10.1002/cyto.990110313
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发表时间：
1990-01-01
期刊：
影响因子：
--
通讯作者：
WAGGONER, AS
中科院分区：
文献类型：
--
作者：
SOUTHWICK, PL;ERNST, LA;WAGGONER, AS
合成了10种新型荧光探针的基础染料--羧甲基吲哚菁染料，并将其转化为琥珀酰亚胺基活性酯，用于蛋白质或其它含氨基物质的荧光标记。该系列成员的荧光发射最大值范围为575至780 nm。已经获得了亲水性的水溶性试剂，其产生标记的抗体而几乎没有形成沉淀的倾向。所获得的荧光强度高于通过用先前描述的花青异硫氰酸酯标记所产生的荧光强度（Jujumdar等人，Cytometry 10：11-19，1989）。这些试剂的效用已被证明在抗体标记的双色免疫荧光成像的内部结构在哺乳动物细胞和双色流式细胞术实验。使用发色团当量重量（W/Ceq）的值，从定量吸收数据对dey样品计算，建议作为一种援助，在制定标记程序。
Ten carboxymethylindocyanine dyes which form the basis of a new series of fluorescent probes have been synthesized and converted into succinimidyl active esters for fluorescent labeling of proteins or other amino-containing substances. Fluorescence emission maxima for members of the series range from 575 to 780 nm. Hydrophilic, water-soluble reagents have been obtained which yield labeled antibodies with little tendency to form precipitates. The fluorescence intensities achieved are higher than those produced by labeling with the cyanine isothiocyanates described previously (Jujumdar et al., Cytometry 10:11-19, 1989). The utility of these reagents has been demonstrated in antibody labeling for two-color immunfluorescent imaging of internal structures in a mammalian cell and for two-color flow-cytometry experiments. The use of values of chromophore-equivalent weight (W/Ceq), calculated from quantitative absorption data on dey samples, is proposed as an aid in formulating labeling procedures.