X-ray scattering used to map the preferred collagen orientation in the human cornea and limbus
X-ray scattering used to map the preferred collagen orientation in the human cornea and limbus
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DOI：
10.1016/j.str.2004.01.002
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发表时间：
2004-02-01
期刊：
影响因子：
5.7
通讯作者：
Meek, KM
中科院分区：
文献类型：
--
作者：
Aghamohammadzadeh, H;Newton, RH;Meek, KM
结缔组织的许多特性是由胶原蛋白的组织决定的。例如，角膜和角膜缘（角膜和巩膜交汇处）中的胶原蛋白组织是角膜曲率以及眼睛聚焦能力的重要决定因素。我们使用同步加速器 X 射线散射来绘制整个人类角膜、角膜缘和邻近巩膜中胶原纤维的方向。我们证明了胶原蛋白在垂直和水平方向上的优选取向，该方向保持在距角膜缘约 1 毫米的范围内，其中发生原纤维的圆形或切向布置。这些数据还用于绘制组织不同部分中总胶原蛋白和优先排列胶原蛋白的相对分布，揭示了相当大的各向异性。提供的详细结构信息是了解组织形状和机械性能的重要一步。
Many properties of connective tissues are governed by the organization of the constituent collagen. For example, the organization of collagen in the cornea and the limbus, where the cornea and sclera meet, is an important determinant of corneal curvature and hence of the eye's focusing power. We have used synchrotron X-ray scattering to map the orientation of the collagen fibrils throughout the human cornea, limbus, and adjacent sclera. We demonstrate a preferred orientation of collagen in the vertical and horizontal directions that is maintained to within about 1 mm from the limbus, where a circular or tangential disposition of fibrils occurs. The data are also used to map the relative distribution of both the total and the preferentially aligned collagen in different parts of the tissue, revealing considerable anisotropy. The detailed structural information provided is an important step toward understanding the shape and the mechanical properties of the tissue.