Feeder-Free Derivation of Induced Pluripotent Stem Cells From Human Immature Dental Pulp Stem Cells
Feeder-Free Derivation of Induced Pluripotent Stem Cells From Human Immature Dental Pulp Stem Cells
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DOI：
10.3727/096368911x566235
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发表时间：
2011-01-01
影响因子：
3.3
通讯作者：
Kerkis, Irina
中科院分区：
文献类型：
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作者：
Beltrao-Braga, Patricia C. B.;Pignatari, Graciela C.;Kerkis, Irina
诱导多能干细胞（iPSCs）可通过在成纤维细胞或其他体细胞类型中强制表达某些基因来创建，使其逆转为与胚胎干细胞（ESC）相似的多能状态。在此，我们使用人类未成熟牙髓干细胞（hIDPSCs）作为创建iPSC的替代来源。hIDPSCs可轻易从年轻和成年患者的易获取组织中分离出来。hIDPSCs具有成纤维细胞样形态，保留了成体多能干细胞的特征。hIDPSCs的重编程速度很快，即使在无饲养层条件下也能产生原代hIDPSC - iPSC集落。hIDPSCs获得了类似ESC的形态，表达多能性标志物，具有稳定、正常的核型，并显示出在体外和体内分化的能力。我们的数据表明，hIDPSCs - iPSCs为未来的细胞治疗和基础研究提供了一个有利的细胞系统，特别是作为儿童发育障碍的模型。
Induced pluripotent stem cells (iPSCs) can be created by forcing expression of certain genes in fibroblasts or other somatic cell types, reversing them to a pluripotent state similar to that of embryonic stem cells (ESC). Here, we used human immature dental pulp stem cells (hIDPSCs) as an alternative source for creating iPSC. hIDPSCs can be easily isolated from accessible tissue of young and adult patients. hIDPSCs possess a fibroblast-like morphology, retaining characteristics of adult multipotent stem cells. Reprogramming of hIDPSCs was fast, producing primary hIDPSC-iPSC colonies even under feeder-free conditions. hIDPSCs acquired ESC-like morphology, expressed pluripotent markers, possessed stable, normal karyotypes, and demonstrated the ability to differentiated in vitro and in vivo. Our data demonstrate that hIDPSCs-iPSCs offer an advantageous cell system for future cell therapy and basic studies, particularly as a model for pediatric developmental disorders.