Modulation of ATP-sensitive K+ channel by insulin in rat skeletal muscle fibers.
Modulation of ATP-sensitive K+ channel by insulin in rat skeletal muscle fibers.
复制标题
胰岛素对大鼠骨骼肌纤维中 ATP 敏感 K 通道的调节。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
D. Camerino
中科院分区：
文献类型：
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作者：
D. Tricarico;Rosanna Mallamaci;M. Barbieri;D. Camerino
在本研究中，我们评估了胰岛素对肌膜ATP敏感性K+通道的调节。对大鼠“体内”给予胰岛素导致骨骼肌纤维超极化。这种作用通过肌肉与格列本脲（一种ATP敏感性K+通道阻滞剂）的“体外”孵育来拮抗。膜片钳实验表明，胰岛素增强ATP敏感性K+通道的平均电流的一个因素的1.4。这种效应是由通道开放概率的增加介导的，而在单通道电导也没有发生变化，也没有在通道密度。在治疗的大鼠中，通道对ATP和格列本脲的敏感性异常降低。我们的结果与胰岛素激活ATP敏感性K+通道一致。这有助于骨骼肌纤维的超极化。
In the present study we evaluated the modulation of the sarcolemmal ATP sensitive K+ channel by insulin. The "in vivo" administration of insulin to the rats led to an hyperpolarization of the skeletal muscle fibers. This effect is antagonized by "in vitro" incubation of the muscle with glybenclamide, an ATP sensitive K+ channel blocker. Patch clamp experiments revealed that insulin enhanced the mean current of the ATP sensitive K+ channel by a factor of 1.4. This effect is mediated by an increase of the channel open probability, while no change occurred in the single channel conductance nor in the channel density. In the treated rats, the sensitivity of the channel to ATP and glybenclamide is abnormally reduced. Our results are consistent with an activation of the ATP sensitive K+ channel by insulin. This contributes to the hyperpolarization of the skeletal muscle fibers.