The inhibition of fluorescence resonance energy transfer between quantum dots for glucose assay.
The inhibition of fluorescence resonance energy transfer between quantum dots for glucose assay.
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DOI：
10.1016/j.bios.2011.11.031
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发表时间：
2012-02
影响因子：
12.6
通讯作者：
Bo Hu;Li-pei Zhang;Mei-Ling Chen;Mingli Chen;Jianhua Wang
中科院分区：
文献类型：
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作者：
Bo Hu;Li-pei Zhang;Mei-Ling Chen;Mingli Chen;Jianhua Wang
两个不同尺寸的量子点之间的荧光共振能量转移（FRET）导致荧光猝灭。因此，提出了结合位点预封闭方法来避免这种影响。葡萄糖在供体上的预结合占据了结合位点，从而阻断了两个量子点之间的共振能量转移，保护荧光不被淬灭。基于这种方法开发了葡萄糖测定法。葡萄糖含量与不存在和存在葡萄糖时的荧光差异相关。在实际应用中，绿色QDs-Con A偶联物作为供体，红色QDs-NH 2-glu偶联物作为受体，形成FRET系统。然后在葡萄糖存在下测量荧光猝灭的抑制。方法的线性范围为0.1-2.0mmolL-1沿着检出限为0.03mmolL-1，相对标准偏差为2.1%（1.0mmolL-1）。血清和尿液样品中91-105%的葡萄糖被回收。值得一提的是，本发明的葡萄糖测定方法还产生荧光色差成像，并且彩色显示器通过视觉检测清楚地识别葡萄糖含量，具有约100%的区分能力。0.5mmolL−1。本方法可以潜在地用于生物样品中的葡萄糖的临床测定，其可以进一步开发成葡萄糖传感器。
Fluorescence resonance energy transfer (FRET) between two quantum dots of different sizes causes fluorescence quenching. Hereby a binding site pre-blocking approach is proposed to avoid this effect. Pre-binding of glucose on the donor occupies the binding sites and thus blocks resonance energy transfer between the two quantum dots, protecting the fluorescence from being quenched. A glucose assay is developed based on this approach. The glucose content is correlated with the fluorescence difference in the absence and in the presence of glucose. In practice, Green QDs–Con A conjugates are used as donors and Red QDs–NH2-glu conjugates as acceptors to form FRET system. The inhibition of fluorescence quenching is then measured in the presence of glucose. A linear calibration graph is achieved within 0.1–2.0mmolL−1, along with a detection limit of 0.03mmolL−1and a RSD of 2.1% (1.0mmolL−1). 91–105% of glucose in serum and urine samples is recovered. It is worth mentioning that the present glucose assay approach also generates a fluorescence chromatic difference imaging, and the color display clearly identifies the glucose contents by visual detection with a distinguishing ability of ca. 0.5mmolL−1. The present approach can potentially be used for the clinical determination of glucose in biological samples which can be further developed into a glucose sensor.